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p90 RSK1 (phospho Thr348) antibody

Anti-p90 RSK1 (phospho Thr348) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50169
Anti-p90 RSK1 (phospho Thr348) antibody used in Western Blot (WB). GTX50169
Anti-p90 RSK1 (phospho Thr348) antibody used in IHC (Paraffin sections) (IHC-P). GTX50169

Cat No. GTX50169

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Peptide sequence around phosphorylation site of threonine 348 (S-R-T(p)-P-R) derived from mouse p90 RSK1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ribosomal protein S6 kinase A1 , HU-1 , MAPKAPK1A , RSK , RSK1 , p90Rsk

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-p90 RSK1 (phospho Thr348) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50169

GTX50169 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50169 p90 RSK1 (phospho Thr348) antibody.

Anti-p90 RSK1 (phospho Thr348) antibody used in Western Blot (WB). GTX50169

GTX50169 WB Image

WB analysis of extracts from HeLa cells untreated or treated with UV using GTX50169 p90 RSK1 (phospho Thr348) antibody.

Anti-p90 RSK1 (phospho Thr348) antibody used in IHC (Paraffin sections) (IHC-P). GTX50169

GTX50169 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50169 p90 RSK1 (phospho Thr348) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf