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pan Cadherin antibody [CH-19]

Anti-pan Cadherin antibody [CH-19] used in IHC (Paraffin sections) (IHC-P). GTX26528
Anti-pan Cadherin antibody [CH-19] used in Western Blot (WB). GTX26528
Anti-pan Cadherin antibody [CH-19] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26528
Anti-pan Cadherin antibody [CH-19] used in Western Blot (WB). GTX26528

Cat. No. GTX26528

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 CH-19

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Rabbit, Goat, Sheep, Bovine, Cat, Dog, Hamster, Chicken, Guinea pig, Pig, Frog, Psammomys, Snake
Package
100 μl ($439)
See all pan Cadherin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:500
IHC-P 1:500
Not tested in other applications.

Positive Control

MDBK

Product Note

Detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, stains adherens type cell-cell junctions regardless of their cadherin type.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Chicken

Immunogen

synthetic peptide corresponding to the C-terminal amino acids of chicken N-Cadherin with an extra N-terminal lysine residue (24 amino acids) coupled to KLH.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_385268

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.

Research Area

DATA IMAGES

Anti-pan Cadherin antibody [CH-19] used in IHC (Paraffin sections) (IHC-P). GTX26528

GTX26528 IHC-P Image

IHC-P analysis of chicken heart tissue using GTX26528 pan Cadherin antibody [CH-19] at 1:500.

Anti-pan Cadherin antibody [CH-19] used in Western Blot (WB). GTX26528

GTX26528 WB Image

WB analysis of MDBK cell lysate using GTX26528 pan Cadherin antibody [CH-19] at 1:2000.

Anti-pan Cadherin antibody [CH-19] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26528

GTX26528 ICC/IF Image

ICC/IF analysis of MDBK cells using GTX26528 pan Cadherin antibody [CH-19] at 1:500(green) with DAPI(blue). Cells were fixed and permeabilized with cold methanol followed by acetone.

Anti-pan Cadherin antibody [CH-19] used in Western Blot (WB). GTX26528

GTX26528 WB Image

WB analysis of (1) C2C12 (2) HeLa (3) NIH-3T3 (4) MDBK (5) COS-7 cell lysate using GTX26528 pan Cadherin antibody [CH-19] at 1:1,000.

REFERENCE

Application Reference

Hsu CW et al. Brain Sci 2021; 11 (12) Changes in the Orexin System in Rats Exhibiting Learned Helplessness Behaviors.
Shimada N et al. ACS Appl Mater Interfaces 2016; (Epub) Reversible monolayer/spheroid cell culture switching by UCST-type thermoresponsive ureido polymers.
Application : ICC/IF
Reactivity : Mouse
Labbe A et al. Invest Ophthalmol Vis Sci 2013; 54 (8) : 5144-50 Corneal nerve structure and function in patients with non-sjogren dry eye: clinical correlations.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 439