*Optimal dilutions/concentrations should be determined by the researcher.
Application
Recommended Dilution
WB
Assay dependent
ICC/IF
1:500
IHC-P
1:500
Not tested in other applications.
Positive Control
MDBK
Product Note
Detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, stains adherens type cell-cell junctions regardless of their cadherin type.
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Chicken
Immunogen
synthetic peptide corresponding to the C-terminal amino acids of chicken N-Cadherin with an extra N-terminal lysine residue (24 amino acids) coupled to KLH.
Purification
Unpurified
Conjugation
Unconjugated
RRID
AB_385268
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Background
This product is useful for the detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, and for demonstration of adherens type cell-cell junctions regardless of their cadherin type.
WB analysis of (1) C2C12 (2) HeLa (3) NIH-3T3 (4) MDBK (5) COS-7 cell lysate using GTX26528 pan Cadherin antibody [CH-19] at 1:1,000.
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GTX26528 IHC-P Image
IHC-P analysis of chicken heart tissue using GTX26528 pan Cadherin antibody [CH-19] at 1:500.
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GTX26528 WB Image
WB analysis of MDBK cell lysate using GTX26528 pan Cadherin antibody [CH-19] at 1:2000.
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GTX26528 ICC/IF Image
ICC/IF analysis of MDBK cells using GTX26528 pan Cadherin antibody [CH-19] at 1:500(green) with DAPI(blue). Cells were fixed and permeabilized with cold methanol followed by acetone.