pan Cadherin antibody

Name: pan Cadherin antibody
Brand: GeneTex
SKU: GTX132646
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-pan Cadherin antibody used in Western Blot (WB). GTX132646

Anti-pan Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132646

Cat. No. GTX132646

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, FACS
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all pan Cadherin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
FACS	Assay dependent

Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

A431 , MCF-7

Predict Reactivity

Rat, Zebrafish, Bovine, Dog, Chicken, Japanese Medaka, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human pan Cadherin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886698

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq]

Research Area

DATA IMAGES

GTX132646 WB Image

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with pan Cadherin antibody (GTX132646) diluted at 1:4000.

GTX132646 ICC/IF Image

pan Cadherin antibody detects pan Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: pan Cadherin stained by pan Cadherin antibody (GTX132646) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

GTX132646 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with pan Cadherin antibody (GTX132646) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX132646 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with pan Cadherin antibody (GTX132646) diluted at 1:5000.

GTX132646 ICC/IF Image

pan Cadherin antibody detects pan Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in ice-cold MeOH for 5 min.
Green: pan Cadherin protein stained by pan Cadherin antibody (GTX132646) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Hsiao-Chin Chou et al. J Biomed Sci 2022; 29 (1) : 40 DUSP3 regulates phosphorylation-mediated degradation of occludin and is required for maintaining epithelial tight junction.

Application : WB, ICC/IF

Reactivity : Mouse

Hung YH et al. Am J Cancer Res 2022; 12 (2) : 713-728 Semaphorin 4C promotes motility and immunosuppressive activity of cancer cells via CRMP3 and PD-L1.

Application : FACS

Reactivity : Human

Hart V et al. J Cancer Res Clin Oncol 2021; HER2-PI9 and HER2-I12: two novel and functionally active splice variants of the oncogene HER2 in breast cancer.

Application : WB

Reactivity : Human

Guo YC et al. Stem Cell Res Ther 2018; 9 (1) : 281 Interleukin-1β induces CXCR3-mediated chemotaxis to promote umbilical cord mesenchymal stem cell transendothelial migration.

Application : WB

Reactivity : Human

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