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pan Cadherin antibody

Anti-pan Cadherin antibody used in Western Blot (WB). GTX132646
Anti-pan Cadherin antibody used in Western Blot (WB). GTX132646
Anti-pan Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132646
Anti-pan Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132646

Cat No. GTX132646

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

A431 , MCF-7

Predict Reactivity

Mouse, Rat, Bovine, Dog, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-termius region of N-Cadherin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq]

Research Area

DATA IMAGES
Anti-pan Cadherin antibody used in Western Blot (WB). GTX132646

GTX132646 WB Image

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with pan Cadherin antibody (GTX132646) diluted at 1:4000.

Anti-pan Cadherin antibody used in Western Blot (WB). GTX132646

GTX132646 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with pan Cadherin antibody (GTX132646) diluted at 1:5000.

Anti-pan Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132646

GTX132646 ICC/IF Image

pan Cadherin antibody detects pan Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in ice-cold MeOH for 5 min.
Green: pan Cadherin protein stained by pan Cadherin antibody (GTX132646) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-pan Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132646

GTX132646 ICC/IF Image

pan Cadherin antibody detects pan Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: pan Cadherin stained by pan Cadherin antibody (GTX132646) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE
REVIEW

pan Cadherin antibody Cat No. GTX132646

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 03-Jan-2019
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : MIA PaCa-2 cells
Blocking : 3% BSA, 25°C, 0.3Hr
Fixation : 4% paraformaldehyde
Permeabilization /Antigen retrieval : 0.8% Triton X-100 in 3% BSA
Primary Antibody : 1:100, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169