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pan Cytokeratin antibody [C-11] (APC)

Anti-pan Cytokeratin antibody [C-11] (APC) used in Flow cytometry (FACS). GTX80205

Cat. No. GTX80205

Reference 15

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 C-11

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse, Rat, Guinea pig
Package
100 μg ($399)
See all pan Cytokeratin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Product Note

This antibody reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Keratin-enriched preparation from human epidermoid carcinoma cell line A431.

Purification

Purified by size-exclusion chromatography

Conjugation

Allophycocyanin (APC)

RRID

AB_11171332

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-pan Cytokeratin antibody [C-11] (APC) used in Flow cytometry (FACS). GTX80205

GTX80205 FACS Image

FACS (intracellular staining) analysis of HeLa cells using GTX80205 pan Cytokeratin antibody [C-11] (APC).

REFERENCE

Application Reference

Labib M et al. Nat Biomed Eng 2020; (Epub) Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting.
Application : ICC/IF
Reactivity : Human

Clone Reference

Michael E B FitzPatrick et al. Cell Rep 2021; 34 (3):108661 Human intestinal tissue-resident memory T?cells comprise transcriptionally and functionally distinct subsets.
Franken A et al. Cancers (Basel) 2021; 13 (23) Multiparametric Circulating Tumor Cell Analysis to Select Targeted Therapies for Breast Cancer Patients.
Ng WL et al. Int J Bioprint 2021; 7 (2):332 Fabrication and Characterization of 3D Bioprinted Triple-layered Human Alveolar Lung Models.
Campello E et al. Dig Liver Dis 2021; (Epub) Acute kidney injury is associated with increased levels of circulating microvesicles in patients with decompensated cirrhosis.
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REVIEW

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Application Reference
Labib M et al. Nat Biomed Eng 2020; (Epub) Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting.
Application : ICC/IF
Reactivity : Human
Labib M et al. Nat Chem 2018; 10 (5):489-495 Single-cell mRNA cytometry via sequence-specific nanoparticle clustering and trapping.
Application : ICC/IF
Reactivity : Human
Green BJ et al. ACS Appl Mater Interfaces 2017; 9 (24):20435-20443 Isolation of Phenotypically Distinct Cancer Cells Using Nanoparticle-Mediated Sorting.
Application : ICC/IF, FACS
Reactivity : Human
Besant JD et al. Nanoscale 2015; 7 (14):6278-85 Velocity valleys enable efficient capture and spatial sorting of nanoparticle-bound cancer cells.
Application : WB
Reactivity : Human
Clone Reference
Michael E B FitzPatrick et al. Cell Rep 2021; 34 (3):108661 Human intestinal tissue-resident memory T?cells comprise transcriptionally and functionally distinct subsets.
Franken A et al. Cancers (Basel) 2021; 13 (23) Multiparametric Circulating Tumor Cell Analysis to Select Targeted Therapies for Breast Cancer Patients.
Ng WL et al. Int J Bioprint 2021; 7 (2):332 Fabrication and Characterization of 3D Bioprinted Triple-layered Human Alveolar Lung Models.
Campello E et al. Dig Liver Dis 2021; (Epub) Acute kidney injury is associated with increased levels of circulating microvesicles in patients with decompensated cirrhosis.
Franken A et al. J Mol Diagn 2019; (Epub) Detection of ESR1 Mutations in Single Circulating Tumor Cells on Estrogen Deprivation Therapy but Not in Primary Tumors from Metastatic Luminal Breast Cancer Patients.
Szczerba BM et al. Nature 2019; (Epub) Neutrophils escort circulating tumour cells to enable cell cycle progression.
Blassl C et al. Mol Oncol 2016; (Epub) Gene expression profiling of single circulating tumor cells in ovarian cancer - Establishment of a multi-marker gene panel.
Neumann MH et al. Biotechnol Prog 2016; (Epub) Isolation and characterization of circulating tumor cells using a novel workflow combining the CellSearchR system and the CellCelector?.
Chebouti I et al. Oncotarget 2016; (Epub) Analysis of disseminated tumor cells before and after platinum based chemotherapy in primary ovarian cancer. Do stem cell like cells predict prognosis?
Schneck H et al. PLoS One 2015; 10 (12):e0144535 EpCAM-Independent Enrichment of Circulating Tumor Cells in Metastatic Breast Cancer.
Ordonez NG et al. Hum Pathol 2013; 44 (7):1195-215 Broad-spectrum immunohistochemical epithelial markers: a review.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399