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pan Cytokeratin antibody [C-11] (FITC)

Anti-pan Cytokeratin antibody [C-11] (FITC) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11212

Cat. No. GTX11212

Reference 15

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 C-11

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Bovine, Frog, Kangaroo rat
Package
100 μl ($339)
See all pan Cytokeratin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:50
IHC-P 1:50
FACS Assay dependent
Not tested in other applications.

Positive Control

human placenta, PtK2

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

keratin-enriched preparation from cultured human epidermoid carcinoma cell line A431.

Purification

Purified immunoglobulin

Conjugation

Fluorescein isothiocyanate (FITC)

RRID

AB_374066

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

GT211212 is a broad spectrum antibody which reacts specifically with a wide variety of normal, reactive and neoplastic epithelial tissues. The antibody reacts with simple, cornifying and non-cornifying squamous epithelia and pseudostratified epithelia. It does not react with non-epithelial normal human tissues. This antibody can be applied to methanol- or acetone-fixed frozen sections, and to formalin-fixed, paraffin-embedded human tissues. Increased staining intensity is seen following proteolytic treatment of tested tissue (protease iunmaskingi). Similarly embedded methacarn-fixed material is also suitable for cytokeratin demonstration. Anti-Pan Cytokeratin exhibits a wide interspecies cross-reactivity down to xenopus laevis (e.g., human, bovine, rat, and frog). GT211212 may be used for the localization of cytokeratins using various immunochemical assays including direct immunofluorescence using frozen or fixed tissue sections or cultured cells. It may also be used in flow cytometry of normal and neoplastic epithelial cells. Intermediate-sized filaments are abundant cytoplasmic structural proteins in most vertebrate cells.

Research Area

DATA IMAGES

Anti-pan Cytokeratin antibody [C-11] (FITC) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11212

GTX11212 ICC/IF Image

The data was published in the journal PLoS One in 2015.PMID: 26695635

REFERENCE

Application Reference

Michael E B FitzPatrick et al. Cell Rep 2021; 34 (3) : 108661 Human intestinal tissue-resident memory T?cells comprise transcriptionally and functionally distinct subsets.
Application : FACS
Reactivity : Human
Franken A et al. Cancers (Basel) 2021; 13 (23) Multiparametric Circulating Tumor Cell Analysis to Select Targeted Therapies for Breast Cancer Patients.
Application : ICC/IF
Reactivity : Human
Ng WL et al. Int J Bioprint 2021; 7 (2) : 332 Fabrication and Characterization of 3D Bioprinted Triple-layered Human Alveolar Lung Models.

Clone Reference

Campello E et al. Dig Liver Dis 2021; (Epub) Acute kidney injury is associated with increased levels of circulating microvesicles in patients with decompensated cirrhosis.
Labib M et al. Nat Biomed Eng 2020; (Epub) Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting.
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REVIEW

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Application Reference
Michael E B FitzPatrick et al. Cell Rep 2021; 34 (3):108661 Human intestinal tissue-resident memory T?cells comprise transcriptionally and functionally distinct subsets.
Application : FACS
Reactivity : Human
Franken A et al. Cancers (Basel) 2021; 13 (23) Multiparametric Circulating Tumor Cell Analysis to Select Targeted Therapies for Breast Cancer Patients.
Application : ICC/IF
Reactivity : Human
Ng WL et al. Int J Bioprint 2021; 7 (2):332 Fabrication and Characterization of 3D Bioprinted Triple-layered Human Alveolar Lung Models.
Franken A et al. J Mol Diagn 2019; (Epub) Detection of ESR1 Mutations in Single Circulating Tumor Cells on Estrogen Deprivation Therapy but Not in Primary Tumors from Metastatic Luminal Breast Cancer Patients.
Application : ICC/IF
Reactivity : Human
Blassl C et al. Mol Oncol 2016; (Epub) Gene expression profiling of single circulating tumor cells in ovarian cancer - Establishment of a multi-marker gene panel.
Application : FACS
Reactivity : Human
Neumann MH et al. Biotechnol Prog 2016; (Epub) Isolation and characterization of circulating tumor cells using a novel workflow combining the CellSearchR system and the CellCelector?.
Reactivity : Human
Schneck H et al. PLoS One 2015; 10 (12):e0144535 EpCAM-Independent Enrichment of Circulating Tumor Cells in Metastatic Breast Cancer.
Clone Reference
Campello E et al. Dig Liver Dis 2021; (Epub) Acute kidney injury is associated with increased levels of circulating microvesicles in patients with decompensated cirrhosis.
Labib M et al. Nat Biomed Eng 2020; (Epub) Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting.
Szczerba BM et al. Nature 2019; (Epub) Neutrophils escort circulating tumour cells to enable cell cycle progression.
Labib M et al. Nat Chem 2018; 10 (5):489-495 Single-cell mRNA cytometry via sequence-specific nanoparticle clustering and trapping.
Green BJ et al. ACS Appl Mater Interfaces 2017; 9 (24):20435-20443 Isolation of Phenotypically Distinct Cancer Cells Using Nanoparticle-Mediated Sorting.
Chebouti I et al. Oncotarget 2016; (Epub) Analysis of disseminated tumor cells before and after platinum based chemotherapy in primary ovarian cancer. Do stem cell like cells predict prognosis?
Besant JD et al. Nanoscale 2015; 7 (14):6278-85 Velocity valleys enable efficient capture and spatial sorting of nanoparticle-bound cancer cells.
Ordonez NG et al. Hum Pathol 2013; 44 (7):1195-215 Broad-spectrum immunohistochemical epithelial markers: a review.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339