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pan Cytokeratin antibody [C-11]

Anti-pan Cytokeratin antibody [C-11] used in Western Blot (WB). GTX48825
Anti-pan Cytokeratin antibody [C-11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48825
Anti-pan Cytokeratin antibody [C-11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48825

Cat No. GTX48825

Host

Mouse

Clonality

Monoclonal

Clone Name

C-11

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IP, ELISA, IHC

Reactivity

Human
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:50 - 1:200
ICC/IF 1:50 - 1:200
IHC-P Assay dependent
IP 1:100
ELISA 1:5000 - 1:20000
IHC 1:50 - 1:200
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts with keratin (56 kDa), keratin 17 (46 kDa), keratin 18 (45 kDa) and keratin 19 (40 kDa) derived from humans.

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This protein A purified monoclonal antibody was produced by repeated immunizations with purified human cytoskeletal preparations from A431 cells.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

keratin 6C , K6E , KRT6E , PPKNEFD

Background

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (i.e. skin) and non-keratinizing cells (i.e. mesothelial)

Database

Research Area

DATA IMAGES

Anti-pan Cytokeratin antibody [C-11] used in Western Blot (WB). GTX48825

GTX48825 WB Image

Western blot using GeneTex Immunochemical's Mouse Mab-anti-Keratin antibody. This antibody recognizes a single 56 kDa band corresponding to human keratin as confirmed by the position of molecular weight markers (not shown). Approximatley 100 μg of keratin from human epidermis was applied under reducing conditions. 1:400 dilution of Mab anti-Keratin was used for 2h followed by detection using a 1:5,000 dilution of IRDyeTM800 conjugated Goat anti-Mouse IgG [H&L] and visualization using the Odyssey® Infrared Imaging System developed by LI-COR.

Anti-pan Cytokeratin antibody [C-11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48825

GTX48825 ICC/IF Image

Immunofluorescence microscopy using GeneTex Immunochemical's Mouse Mab-anti-Keratin antibody. Confocal slices of HeLa cells are between 0.5 and 0.6 um where the image is taken near the bottom of the cell. Use FITC a 1:2,000 dilution of FITC-conjugated Goat anti-Mouse IgG [H&L] for detection.

Anti-pan Cytokeratin antibody [C-11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48825

GTX48825 ICC/IF Image

Immunofluorescence Microscopy of GeneTex Anti-Keratin antibody (GTX48825) was used with GeneTex Dylight 488 goat anti-mouse (shown in green) to detect Keratin by Immunofluorescence. In the same experiment, GeneTex polyclonal Anti-HDAC-1 antibody was used with Anti-Rabbit IgG (shown in red) to detect HDAC-1.

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf