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pan Cytokeratin antibody [KRT/1877R]

Anti-pan Cytokeratin antibody [KRT/1877R] used in IHC (Paraffin sections) (IHC-P). GTX02756
Anti-pan Cytokeratin antibody [KRT/1877R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02756

Cat. No. GTX02756

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 KRT/1877R

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Goat, Bovine, Guinea pig, Pig, Monkey, Frog
Package
100 μg ($529)
See all pan Cytokeratin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2 μg/ml
ICC/IF 1-2 μg/ml
IHC-P 1-2 μg/ml
FACS 1-2 μg/million cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Product Note

This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13) and 45kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Keratin-enriched preparation from cultured human epithelial cells

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-pan Cytokeratin antibody [KRT/1877R] used in IHC (Paraffin sections) (IHC-P). GTX02756

GTX02756 IHC-P Image

IHC-P analysis of human skin tissue section using GTX02756 pan Cytokeratin antibody [KRT/1877R].

Anti-pan Cytokeratin antibody [KRT/1877R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02756

GTX02756 ICC/IF Image

ICC/IF analysis of MeOH-fixed MCF-7 cells using GTX02756 pan Cytokeratin antibody [KRT/1877R].
Green : Primary antibody
Red : nuclear counterstain

REFERENCE

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REVIEW

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Package List Price ($)
$ 529