PRODUCT
Summary
Pan-Cytokeratin antibody [PCK-26] is a mouse monoclonal antibody that detects multiple cytokeratins (i.e., Type II cytokeratins 1, 5, 6, and 8), a group of intermediate filament proteins present in most human epithelial tissues. This reagent is a broad spectrum antibody that reacts with a variety of normal, reactive, and neoplastic epithelial tissues, as well as simple, cornifying, and non-cornifying squamous epithelia and pseudostratified epithelia.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
1:300 |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Product Note
The antibody recognizes an epitope located on the Type II cytokeratins 1, 5, 6, and 8. PCK-26 is a broad spectrum antibody which reacts specifically with a variety of normal, reactive, and neoplastic epithelial tissues. The antibody reacts with simple, cornifying, and non-cornifying squamous epithelia and pseudostratified epithelia.
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
cytokeratin from human epidermis.
Purification
Unpurified
Conjugation
Unconjugated
RRID
AB_385223
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Background
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratin 5, 6 and 8 are members of the type II neutral-to-basic subfamily. Monoclonal antibodies to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. PCK-26 facilitates the typing of normal, metaplastic and neoplastic cells and it may aid in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micro-metastases in lymph nodes and other tissues and for determining the origin of poorly differentiated tumors (1,2).
Research Area
DATA IMAGES
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GTX26401 IHC-P Image
IHC-P analysis of human placenta sections using GTX26401 pan Cytokeratin antibody [PCK-26] at 1:300.
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GTX26401 WB Image
WB analysis of HeLa cell lysate using GTX26401 pan Cytokeratin antibody[PCK-26].
Lane 1 : Antibody dilution 1:300 Lane 2 : Antibody dilution 1:500 Lane 3 : Antibody dilution 1:1000.
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REFERENCE
REVIEW
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