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pan-HP1 antibody - ChIP grade

Anti-pan-HP1 antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60833
Anti-pan-HP1 antibody - ChIP grade used in Western Blot (WB). GTX60833
Anti-pan-HP1 antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60833

Cat. No. GTX60833

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ChIP assay

Reactivity

 Human, Mouse
Package
50 μg ($429)
See all pan-HP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF 1:500
ChIP assay 4 μg
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human HP1 β (Heterochromatin protein 1 homolog beta), using the full length recombinant GST tagged protein. The antibody also recognizes the α and γ isoforms.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chromobox 1 , chromobox 3 , chromobox 5 , CBX , HP1-BETA , HP1Hs-beta , HP1Hsbeta , M31 , MOD1 , p25beta , HECH , HP1-GAMMA , HP1Hs-gamma , HEL25 , HP1 , HP1A

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-pan-HP1 antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60833

GTX60833 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60833 pan-HP1 antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:500

Anti-pan-HP1 antibody - ChIP grade used in Western Blot (WB). GTX60833

GTX60833 WB Image

WB analysis of nuclear extracts (20 μg) from HeLa cells using GTX60833 pan-HP1 antibody - ChIP grade.
Dilution : 1:1,000

Anti-pan-HP1 antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60833

GTX60833 ChIP assay Image

ChIP analysis of NIH3T3 cells using GTX60833 pan-HP1 antibody - ChIP grade. QPCR was performed on the IP'd DNA with optimized primer sets for the rDNA promoter and for a subtelomeric sequence of chromosome 19. This figure shows the relative enrichment as compared to a no antibody negative control ChIP.
Antibody amount : 4μg

REFERENCE

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REVIEW

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Package List Price ($)
$ 429