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pan NOS antibody [NOS-3F7-B11 B5]

Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801
Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Western Blot (WB). GTX22801
Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801
Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801
Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801

Cat No. GTX22801

Host

Mouse

Clonality

Monoclonal

Clone Name

NOS-3F7-B11 B5

Isotype

IgM

Application

WB, ICC/IF, IHC-Fr, IP, IHC

Reactivity

Human, Mouse, Rat, Bovine
Package
200 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:50-1:500
ICC/IF 1:20
IHC-Fr 1:100
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Product Note

This antibody detects brain nitric oxide synthase (bNOS), inducible NOS (iNOS) and epithelial NOS (eNOS).

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Bovine

Immunogen

Purified bovine bNOS.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801

GTX22801 ICC/IF Image

ICC/IF analysis of SK-N-MC cells using GTX22801 pan NOS antibody [NOS-3F7-B11 B5]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC

Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Western Blot (WB). GTX22801

GTX22801 WB Image

WB analysis of 25 ug of mouse brain (lane 1) and C2C12 (lane 2) lysates using GTX22801 pan NOS antibody [NOS-3F7-B11 B5].
Dilution : 1:100

Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801

GTX22801 ICC/IF Image

ICC/IF analysis of SK-N-MC Cells using GTX22801 pan NOS antibody [NOS-3F7-B11 B5]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801

GTX22801 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22801 pan NOS antibody [NOS-3F7-B11 B5]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC

Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801

GTX22801 ICC/IF Image

ICC/IF analysis of C2C12 cells using GTX22801 pan NOS antibody [NOS-3F7-B11 B5]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 379