*Optimal dilutions/concentrations should be determined by the researcher.
Application
Recommended Dilution
WB
1:50-1:500
ICC/IF
1:20
IHC-Fr
1:100
IP
Assay dependent
IHC
Assay dependent
Not tested in other applications.
Product Note
This antibody detects brain nitric oxide synthase (bNOS), inducible NOS (iNOS) and epithelial NOS (eNOS).
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Bovine
Immunogen
Purified bovine bNOS.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
ICC/IF analysis of SK-N-MC cells using GTX22801 pan NOS antibody [NOS-3F7-B11 B5]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:20 overnight at 4ºC
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GTX22801 ICC/IF Image
ICC/IF analysis of C2C12 cells using GTX22801 pan NOS antibody [NOS-3F7-B11 B5]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:20 overnight at 4ºC
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GTX22801 WB Image
WB analysis of 25 ug of mouse brain (lane 1) and C2C12 (lane 2) lysates using GTX22801 pan NOS antibody [NOS-3F7-B11 B5]. Dilution : 1:100
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GTX22801 ICC/IF Image
ICC/IF analysis of SK-N-MC Cells using GTX22801 pan NOS antibody [NOS-3F7-B11 B5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4ºC
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GTX22801 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX22801 pan NOS antibody [NOS-3F7-B11 B5]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:20 overnight at 4ºC
![Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801](/upload/website/prouct_img/small/GTX22801/GTX22801_471_ICC-IF_w_23060620_841.webp "Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801")
![Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801](/upload/website/prouct_img/small/GTX22801/GTX22801_469_ICC-IF_w_23060620_807.webp "Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801")
![Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Western Blot (WB). GTX22801](/upload/website/prouct_img/small/GTX22801/GTX22801_1599_WB_w_23060620_431.webp "Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Western Blot (WB). GTX22801")
![Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801](/upload/website/prouct_img/small/GTX22801/GTX22801_472_ICC-IF_w_23060620_796.webp "Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801")
![Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801](/upload/website/prouct_img/small/GTX22801/GTX22801_470_ICC-IF_w_23060620_118.webp "Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801")
![Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801](/upload/website/prouct_img/normal/GTX22801/GTX22801_471_ICC-IF_w_23060620_841.webp "Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801")
![Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801](/upload/website/prouct_img/normal/GTX22801/GTX22801_469_ICC-IF_w_23060620_807.webp "Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801")
![Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Western Blot (WB). GTX22801](/upload/website/prouct_img/normal/GTX22801/GTX22801_1599_WB_w_23060620_431.webp "Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Western Blot (WB). GTX22801")
![Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801](/upload/website/prouct_img/normal/GTX22801/GTX22801_472_ICC-IF_w_23060620_796.webp "Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801")
![Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801](/upload/website/prouct_img/normal/GTX22801/GTX22801_470_ICC-IF_w_23060620_118.webp "Anti-pan NOS antibody [NOS-3F7-B11 B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22801")