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pan Synuclein antibody

Anti-pan Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX133368
Anti-pan Synuclein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133368
Anti-pan Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX133368
Anti-pan Synuclein antibody used in Western Blot (WB). GTX133368
Anti-pan Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133368
Anti-pan Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133368

Cat. No. GTX133368

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all pan Synuclein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Recombinant protein encompassing a sequence within the center region of Rat pan Synuclein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886958

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Snca antibody , synuclein alpha antibody

Cellular Localization

Cytoplasm

Background

may play a role in regulation of synaptic vesicle biogenesis and organization; may be involved in neurotransmission [RGD]

Research Area

DATA IMAGES

Anti-pan Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX133368

GTX133368 IHC-P Image

pan Synuclein antibody detects pan Synuclein protein at cell membrane in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
pan Synuclein antibody (GTX133368) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-pan Synuclein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133368

GTX133368 ICC/IF Image

pan Synuclein antibody detects pan Synuclein protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: pan Synuclein protein stained by pan Synuclein antibody (GTX133368) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-pan Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX133368

GTX133368 IHC-P Image

pan Synuclein antibody detects pan Synuclein protein at cell membrane in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
pan Synuclein antibody (GTX133368) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-pan Synuclein antibody used in Western Blot (WB). GTX133368

GTX133368 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with pan Synuclein antibody (GTX133368) diluted at 1:1000.

Anti-pan Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133368

GTX133368 IHC-Fr Image

alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX133368) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-pan Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133368

GTX133368 IHC-Fr Image

pan Synuclein antibody detects pan Synuclein protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: pan Synuclein protein stained by pan Synuclein antibody (GTX133368) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169