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prM protein (JEV) antibody

Anti-prM protein (JEV) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131833
Anti-prM protein (JEV) antibody used in Western Blot (WB). GTX131833

Cat No. GTX131833

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Japanese encephalitis virus
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

JEV infected BHK-21
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.072 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein encompassing a sequence within the center region of Japanese encephalitis virus JEV prM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-prM protein (JEV) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131833

GTX131833 ICC/IF Image

prM protein (Japanese encephalitis virus) antibody detects prM protein (Japanese encephalitis virus) by immunofluorescence analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis virus (right) were fixed in methanol/acetone.
Green: prM protein (Japanese encephalitis virus) stained by prM protein (Japanese encephalitis virus) antibody (GTX131833) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-prM protein (JEV) antibody used in Western Blot (WB). GTX131833

GTX131833 WB Image

PrM protein (JEV) antibody detects PrM protein (JEV) protein by western blot analysis. Un-infected (-) and infected (+, JEV infection) BHK-21 whole cell extracts (20 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PrM protein (JEV) antibody (GTX131833) at a dilution of 1:1000.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf