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Japanese encephalitis virus prM antibody

Anti-Japanese encephalitis virus prM antibody used in Western Blot (WB). GTX131833
Anti-Japanese encephalitis virus prM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131833

Cat. No. GTX131833

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Japanese encephalitis virus
Package
100 μl ($399),
25 μl ($169)
See all Japanese encephalitis virus prM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

JEV infected BHK-21

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Japanese encephalitis virus

Immunogen

Recombinant protein encompassing a sequence within the center region of prM protein (JEV). (Japanese Encephalitis Virus strain Jaoars982) The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886526

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Japanese encephalitis Virus Premembrane , Japanese encephalitis Virus PrM , JEV Premembrane , JEV PrM

Research Area

DATA IMAGES

Anti-Japanese encephalitis virus prM antibody used in Western Blot (WB). GTX131833

GTX131833 WB Image

PrM protein (JEV) antibody detects PrM protein (JEV) protein by western blot analysis. Un-infected (-) and infected (+, JEV infection) BHK-21 whole cell extracts (20 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PrM protein (JEV) antibody (GTX131833) at a dilution of 1:1000.

Anti-Japanese encephalitis virus prM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131833

GTX131833 ICC/IF Image

prM protein (Japanese encephalitis virus) antibody detects prM protein (Japanese encephalitis virus) by immunofluorescence analysis.
Samples: BHK-21 cells mock (left) and infected with Japanese encephalitis virus (right) were fixed in methanol/acetone.
Green: prM protein (Japanese encephalitis virus) stained by prM protein (Japanese encephalitis virus) antibody (GTX131833) diluted at 1:1000.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Ishida K et al. PLoS Pathog 2023; 19 (10) : e1011681 N-linked glycosylation of flavivirus E protein contributes to viral particle formation.
Liang Z et al. Viruses 2023; 15 (7) Interaction between hTIM-1 and Envelope Protein Is Important for JEV Infection.
Keisuke Tabata et al. J Virol 2021; 95 (15) : e0223420 Endoplasmic Reticulum-Associated Degradation Controls Virus Protein Homeostasis, Which Is Required for Flavivirus Propagation.
Application : WB
Chang YH et al. Viruses 2020; 12 (3) Mosquito Cell-Derived Japanese Encephalitis Virus-Like Particles Induce Specific Humoral and Cellular Immune Responses in Mice.
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169