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pro-EGF antibody [N3C1], Internal

Anti-pro-EGF antibody [N3C1], Internal used in Western Blot (WB). GTX111099
Anti-pro-EGF antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111099

Cat. No. GTX111099

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Positive Control

rat brain

Predict Reactivity

Cat, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human pro-EGF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618248

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epidermal growth factor , HOMG4 , URG

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-pro-EGF antibody [N3C1], Internal used in Western Blot (WB). GTX111099

GTX111099 WB Image

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with pro-EGF antibody [N3C1], Internal (GTX111099) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-pro-EGF antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111099

GTX111099 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using EGF(GTX111099) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for pro-EGF antibody [N3C1], Internal (GTX111099). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for pro-EGF antibody [N3C1], Internal (GTX111099). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169