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proBDNF antibody

Anti-proBDNF antibody used in IHC (Frozen sections) (IHC-Fr). GTX04075
Anti-proBDNF antibody used in Western Blot (WB). GTX04075
Anti-proBDNF antibody used in Western Blot (WB). GTX04075
Anti-proBDNF antibody used in IHC (Frozen sections) (IHC-Fr). GTX04075

Cat. No. GTX04075

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr, IP

Reactivity

 Human, Mouse, Rat
Package
50 μl ($669)
See all BDNF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Product Note

The antibody is specific for proBDNF, it does not crossreact with proNGF, proNT-3 or mature BDNF.

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 1% BSA

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide (C)DEDQKVRPNEENNKDAD, corresponding to amino acid residues 72-88 of human BDNF (precursor) (Accession P23560). Pro-domain of the BDNF protein.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

brain derived neurotrophic factor , ANON2 , BULN2

Cellular Localization

Secreted

Background

This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-proBDNF antibody used in IHC (Frozen sections) (IHC-Fr). GTX04075

GTX04075 IHC-Fr Image

IHC-Fr analysis of rat cerebellum tissue using GTX04075 proBDNF antibody. proBDNF (Red) appears in Purkinje cell bodies (vertical arrows) and in astrocytic processes (horizontal arrows in panel A) but not in Purkinje neuronal dendrites stained for calbindin D28k (Green, in panel B) in the same brain section.

Anti-proBDNF antibody used in Western Blot (WB). GTX04075

GTX04075 WB Image

WB analysis of recombinant proteins using GTX04075 proBDNF antibody.
Lane 1 : 20 ng Human proBDNF
Lane 2 : 200 ng Human proNGF
Lane 3 : 200 ng proNT-3
Lane 4 : 200 ng Human BDNF
Lane 5 : 200 ng Native mouse NGF 2.5S protein
Lane 6 : 200 ng Human Neurotrophin-3 (NT-3)
Dilution : 1:400

Anti-proBDNF antibody used in Western Blot (WB). GTX04075

GTX04075 WB Image

WB analysis of various cell lysates using GTX04075 proBDNF antibody.
Lane 1 : C6 cell lysates
Lane 2 : SH-SY5Y cell lysates
Lane 3 : C6 cell lysates treated with blocking peptide
Lane 4 : SH-SY5Y cell lyates treated with blocking peptide
Dilution : 1:200

Anti-proBDNF antibody used in IHC (Frozen sections) (IHC-Fr). GTX04075

GTX04075 IHC-Fr Image

IHC-Fr analysis of rat hippocampus dentate gyrus tissue using GTX04075 proBDNF antibody.
Panel A : BDNF staining (red) appears in interneuron outlines in the hilus (H) region and in the granule layer (G).
Panel B : proBDNF staining (GTX04075, green) in the same section appears in interneuron outlines in the hilus (H) region and in the granule layer (G).
Dilution : 1:200

REFERENCE

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Package List Price ($)
$ 669