GeneTex
United States (US)

proBNP antibody [13G12]

proBNP-antibody-13G12_Western blot_GTX14712-1_18121411_274.jpg

Cat No. GTX14712

Host Mouse
Clonality Monoclonal
Clone Name 13G12
Isotype IgG2a
Application WB, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

14.73 kDa. ( Note )

Specificity/Sensitivity

NT-proBNP and proBNP
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.09 % sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic N-terminal pro brain natriuretic peptide (NT-proBNP), corresponding to amino acid residues a.a.r. 13-27. The peptides used for immunization were conjugated with carrier protein.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Natriuretic Peptide B,Bnp,Nppb

Cellular Localization

Secreted

Background

This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES
proBNP-antibody-13G12_Western blot_GTX14712-1_18121411_274.jpg

GTX14712 WB Image

Detection of human recombinant proBNP expressed mammalian cells in Western blotting by different monoclonal antibodies after Tricine-SDS gel electrophoresis. Antigen: recombinant proBNP expressed in mammalian cells, 0.15 ug; Lane 1: proBNP antibody [5B6] (GTX13111); Lane 2: proBNP antibody [15F11] (GTX13115); Lane 3: proBNP antibody [3G12] (GTX14712); Lane 4: proBNP antibody [11D1] (GTX13059); Lane 5: proBNP antibody [24E11] (GTX13123); Both proBNP antibody [11D1] (GTX13059) and proBNP antibody [24E11] (GTX13123)do not recognize glycosylated proBNP expressed in mammalian cells.