APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 4 - 6 μg/ml |
| 1:50 - 1:250 |
| 1:10 - 1:500 |
| 1:10 - 1:1000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE was used as immunogen.
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
TNF superfamily member 11 , CD254 , ODF , OPGL , OPTB2 , RANKL , TNLG6B , TRANCE , hRANKL2 , sOdf
Background
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-sRANKL antibody [12A380] used in IHC (Paraffin sections) (IHC-P). GTX12125](/templates/default/js/lightbox/images/loading.gif "Anti-sRANKL antibody [12A380] used in IHC (Paraffin sections) (IHC-P). GTX12125")
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GTX12125 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX12125 sRANKL antibody [12A380].
Dilution : 1:50
Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)
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GTX12125 FACS Image
FACS analysis of mouse splenocytes using GTX12125 sRANKL antibody [12A380].
Green : untreated mouse splenocytes
Red : 72 hour ConA-stimulated mouse splenocytes
Antibody amount : 1μg
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GTX12125 FACS Image
FACS analysis of RAW264.7 cells using GTX12125 sRANKL antibody [12A380].
Purple : Primary antibody
Green : isotype control
Shaded histogram : cell only
Antibody amount : 2μg
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GTX12125 WB Image
WB analysis of recombinant sRANKL (partial) protien using GTX12125 sRANKL antibody [12A380].
Dilution : 1 μg/ml
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REFERENCE
There are currently no references for sRANKL antibody [12A380] (GTX12125). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for sRANKL antibody [12A380] (GTX12125). Be the first to share your experience with this product.
![Anti-sRANKL antibody [12A380] used in IHC (Paraffin sections) (IHC-P). GTX12125](/upload/website/prouct_img/small/GTX12125/GTX12125_387_IHC-P_w_23060519_700.webp "Anti-sRANKL antibody [12A380] used in IHC (Paraffin sections) (IHC-P). GTX12125")
![Anti-sRANKL antibody [12A380] used in Flow cytometry (FACS). GTX12125](/upload/website/prouct_img/small/GTX12125/GTX12125_5_FACS_w_23060519_508.webp "Anti-sRANKL antibody [12A380] used in Flow cytometry (FACS). GTX12125")
![Anti-sRANKL antibody [12A380] used in Flow cytometry (FACS). GTX12125](/upload/website/prouct_img/small/GTX12125/GTX12125_6_FACS_w_23060519_903.webp "Anti-sRANKL antibody [12A380] used in Flow cytometry (FACS). GTX12125")
![Anti-sRANKL antibody [12A380] used in Western Blot (WB). GTX12125](/upload/website/prouct_img/small/GTX12125/GTX12125_915_WB_w_23060519_226.webp "Anti-sRANKL antibody [12A380] used in Western Blot (WB). GTX12125")
![Anti-sRANKL antibody [12A380] used in IHC (Paraffin sections) (IHC-P). GTX12125](/upload/website/prouct_img/normal/GTX12125/GTX12125_387_IHC-P_w_23060519_700.webp "Anti-sRANKL antibody [12A380] used in IHC (Paraffin sections) (IHC-P). GTX12125")
![Anti-sRANKL antibody [12A380] used in Flow cytometry (FACS). GTX12125](/upload/website/prouct_img/normal/GTX12125/GTX12125_5_FACS_w_23060519_508.webp "Anti-sRANKL antibody [12A380] used in Flow cytometry (FACS). GTX12125")
![Anti-sRANKL antibody [12A380] used in Flow cytometry (FACS). GTX12125](/upload/website/prouct_img/normal/GTX12125/GTX12125_6_FACS_w_23060519_903.webp "Anti-sRANKL antibody [12A380] used in Flow cytometry (FACS). GTX12125")
![Anti-sRANKL antibody [12A380] used in Western Blot (WB). GTX12125](/upload/website/prouct_img/normal/GTX12125/GTX12125_915_WB_w_23060519_226.webp "Anti-sRANKL antibody [12A380] used in Western Blot (WB). GTX12125")