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sRANKL antibody [12A380]

Anti-sRANKL antibody [12A380] used in Flow cytometry (FACS). GTX12125
Anti-sRANKL antibody [12A380] used in IHC (Paraffin sections) (IHC-P). GTX12125
Anti-sRANKL antibody [12A380] used in Flow cytometry (FACS). GTX12125
Anti-sRANKL antibody [12A380] used in Western Blot (WB). GTX12125

Cat No. GTX12125

Host

Mouse

Clonality

Monoclonal

Clone Name

12A380

Isotype

IgG1

Application

WB, IHC-P, IHC-Fr, FACS

Reactivity

Human, Mouse
Package
50 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 4 - 6 μg/ml
IHC-P 1:50 - 1:250
IHC-Fr 1:10 - 1:500
FACS 1:10 - 1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE was used as immunogen.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TNF superfamily member 11 , CD254 , ODF , OPGL , OPTB2 , RANKL , TNLG6B , TRANCE , hRANKL2 , sOdf

Background

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-sRANKL antibody [12A380] used in Flow cytometry (FACS). GTX12125

GTX12125 FACS Image

FACS analysis of mouse splenocytes using GTX12125 sRANKL antibody [12A380].
Green : untreated mouse splenocytes
Red : 72 hour ConA-stimulated mouse splenocytes
Antibody amount : 1μg

Anti-sRANKL antibody [12A380] used in IHC (Paraffin sections) (IHC-P). GTX12125

GTX12125 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX12125 sRANKL antibody [12A380].
Dilution : 1:50
Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)

Anti-sRANKL antibody [12A380] used in Flow cytometry (FACS). GTX12125

GTX12125 FACS Image

FACS analysis of RAW264.7 cells using GTX12125 sRANKL antibody [12A380].
Purple : Primary antibody
Green : isotype control
Shaded histogram : cell only
Antibody amount : 2μg

Anti-sRANKL antibody [12A380] used in Western Blot (WB). GTX12125

GTX12125 WB Image

WB analysis of recombinant sRANKL (partial) protien using GTX12125 sRANKL antibody [12A380].
Dilution : 1 μg/ml

REFERENCE

There are currently no references for sRANKL antibody [12A380] (GTX12125). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for sRANKL antibody [12A380] (GTX12125). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319