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sRANKL antibody [R001]

Anti-sRANKL antibody [R001] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX02153

Cat No. GTX02153

Host

Rabbit

Clonality

Monoclonal

Clone Name

R001

Isotype

IgG

Application

Neutralizing/Inhibition

Reactivity

Human
Package
200 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
Neutralizing/Inhibition Assay dependent

Note :

Neutralizing/Inhibition
1. In a functional ELISA which immobilized Human OPG protein (GTX01366-pro) at 2 μg/ml (100 μl/well) in the plate, this antibody can block the binding of 0.2 μg/ml of recombinant Human TNFSF11 protein to Human TNFRSF11A protein or Human TNFRSF11B protein. And the EC₅₀ is 0.48 μg/ml or 1.02 μg/ml, respectively.
2. The neutralization activity of this antibody is measured by its ability to neutralize TNFSF11 induced osteoclast-like cell formation in RAW 264.7 cells. The Neutralization titer (IC₅₀) is typically 4-16 ng/ml in the presence of 30 ng/ml Human RANKL protein.

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Product Note

We do not recommend use of this product for Mouse.

PROPERTIES

Form

Liquid

Buffer

0.2 μm filtered Histidine and Arginine buffer (pH 6.0), 120mM NaCl, 0.02% Tween 80

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Human RANKL / OPGL / TNFSF11 / CD254 protein

Purification

Purified by Protein A

Endotoxin

< 3 EU/mg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TNF superfamily member 11 , CD254 , ODF , OPGL , OPTB2 , RANKL , TNLG6B , TRANCE , hRANKL2 , sOdf

Background

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-sRANKL antibody [R001] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX02153

GTX02153 Neutralizing/Inhibition Image

Osteoclast-like cell formation elicited by recombinant human IL4 protein (0.2 ng/ml) is neutralized by increasing concentrations of GTX02153 sRANKL antibody [R001].
IC₅₀ : 4-16 ng/ml.

REFERENCE

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REVIEW

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Package List Price ($)
$ 329