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sRANKL antibody

Anti-sRANKL antibody used in ELISA (ELISA). GTX10343
Anti-sRANKL antibody used in Western Blot (WB). GTX10343
Anti-sRANKL antibody used in Western Blot (WB). GTX10343

Cat No. GTX10343

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA, sELISA

Reactivity

Mouse
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1 - 0.2 μg/mL
ELISA 0.5 μg/mL
sELISA 0.5 - 2.0 ug/ml

Note :

sELISA
Capture : GTX10343, Detection : Biotinylated Anti-Murine sRANKL antibody

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Specificity/Sensitivity

No crossreactivity with recombinant human CD40 ligand.

PROPERTIES

Form

Liquid

Buffer

0.5X PBS. pH 7.4

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant msRANKL (murine sRANK Ligand).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor necrosis factor (ligand) superfamily, member 11 , Ly109l , ODF , OPGL , RANKL , Trance

Background

TRANCE, also called RANKL (RANK ligand), OPGL (Osteoprotegrin ligand), and ODF (Osteoclast differentiation factor), is a type II transmembrane signaling protein of the TNF superfamily. The extracellular domain contains two potential N-linked glycosylation sites. Mouse TRANCE cDNA encodes a 316 amino acid protein with a calculated molecular mass of approximately 28 kDa. As a result of glycosylation, recombinant mouse TRANCE migrates as an approximately 36 kDa protein in SDS-PAGE. Human and mouse TRANCE share approximately 85% amino acid sequence identity. TRANCE was originally identified as an immediate early gene upregulated by T cell receptor stimulation. It is a key regulator of the immune system and of bone development and homeostasis. Multi-functions of TRANCE include induction of activation of c-jun N-terminal kinase in T cells, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis and lymph node organogenesis. The cell surface signaling receptor of TRANCE (RANKL) is RANK which undergoes receptor clustering during signal transduction. TRANCE is highly expressed in thymus and lymph nodes but not in nonlymphoid tissues.1 It is abundantly expressed in T cells but not in B cells. TRANCE activates mature dendritic cells, inducing cytokine production, suggesting that it is a factor in the T cell dentritic cell interaction during an immune response. TRANCE expression is found on osteoblasts and regulates osteoclast differentiation function.

Database

Research Area

DATA IMAGES

Anti-sRANKL antibody used in ELISA (ELISA). GTX10343

GTX10343 ELISA Image

ELISA analysis of mouse RANKL recombinant protein (0.2 - 0.4 ng/well) using GTX10343 sRANKL antibody(capture antibody) at 0.5 - 2.0 ug/ml and Biotinylated Anti-Murine sRANKL as a detection antibody.

Anti-sRANKL antibody used in Western Blot (WB). GTX10343

GTX10343 WB Image

WB analysis of sequential diluted mouse RANKL recombinant protein under either reducing or non-reducing conditions using GTX10343 sRANKL antibody.
Working concentration : 0.1 - 0.2 ug/ml

Anti-sRANKL antibody used in Western Blot (WB). GTX10343

GTX10343 WB Image

WB analysis of sequential diluted mouse RANKL recombinant protein under either reducing or non-reducing conditions using GTX10343 sRANKL antibody.
Working concentration : 0.1 - 0.2 ug/ml

REFERENCE

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REVIEW

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