APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.1 - 0.2 μg/mL |
0.5 μg/mL |
0.5 - 2.0 ug/ml |
Note :
Sandwich ELISA
Capture : GTX10343, Detection : Biotinylated Anti-Murine sRANKL antibody
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Not tested in other applications.
Calculated MW
Product Note
No crossreactivity with recombinant human CD40 ligand.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant msRANKL (murine sRANK Ligand).
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tumor necrosis factor (ligand) superfamily, member 11 , Ly109l , ODF , OPGL , RANKL , Trance
Background
TRANCE, also called RANKL (RANK ligand), OPGL (Osteoprotegrin ligand), and ODF (Osteoclast differentiation factor), is a type II transmembrane signaling protein of the TNF superfamily. The extracellular domain contains two potential N-linked glycosylation sites. Mouse TRANCE cDNA encodes a 316 amino acid protein with a calculated molecular mass of approximately 28 kDa. As a result of glycosylation, recombinant mouse TRANCE migrates as an approximately 36 kDa protein in SDS-PAGE. Human and mouse TRANCE share approximately 85% amino acid sequence identity. TRANCE was originally identified as an immediate early gene upregulated by T cell receptor stimulation. It is a key regulator of the immune system and of bone development and homeostasis. Multi-functions of TRANCE include induction of activation of c-jun N-terminal kinase in T cells, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis and lymph node organogenesis. The cell surface signaling receptor of TRANCE (RANKL) is RANK which undergoes receptor clustering during signal transduction. TRANCE is highly expressed in thymus and lymph nodes but not in nonlymphoid tissues.1 It is abundantly expressed in T cells but not in B cells. TRANCE activates mature dendritic cells, inducing cytokine production, suggesting that it is a factor in the T cell dentritic cell interaction during an immune response. TRANCE expression is found on osteoblasts and regulates osteoclast differentiation function.
Database
Research Area
DATA IMAGES
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GTX10343 ELISA Image
ELISA analysis of mouse RANKL recombinant protein (0.2 - 0.4 ng/well) using GTX10343 sRANKL antibody(capture antibody) at 0.5 - 2.0 ug/ml and Biotinylated Anti-Murine sRANKL as a detection antibody.
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GTX10343 WB Image
WB analysis of sequential diluted mouse RANKL recombinant protein under either reducing or non-reducing conditions using GTX10343 sRANKL antibody. Working concentration : 0.1 - 0.2 ug/ml
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GTX10343 WB Image
WB analysis of sequential diluted mouse RANKL recombinant protein under either reducing or non-reducing conditions using GTX10343 sRANKL antibody. Working concentration : 0.1 - 0.2 ug/ml
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REFERENCE
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REVIEW
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