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sarcomeric alpha Actinin antibody [EA-53]

Anti-sarcomeric alpha Actinin antibody [EA-53] used in IHC (Paraffin sections) (IHC-P). GTX29465
Anti-sarcomeric alpha Actinin antibody [EA-53] used in IHC (Paraffin sections) (IHC-P). GTX29465

Cat. No. GTX29465

Reference 7
1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 EA-53

Isotype

 IgG1

Application

 ICC/IF, IHC-P, IHC-Fr, IHC-Wm

Reactivity

 Human, Mouse, Zebrafish
Package
500 μl ($399)
See all Sarcomeric Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P 1:20‐1:30
IHC-Fr Assay dependent
IHC-Wm Assay dependent

Note :

IHC-P
Recommendations for antigen retrieval : EDTA buffer pH 8.0 at room temperature for 30 mins.
Not tested in other applications.

Product Note

This antibody is specific for α-skeletal muscle actinin and α-cardiac actinin. It stains Z lines and dots in stress fibers of myotubes in skeletal and cardiac muscle but not in non-sarcomeric muscle elements.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Antigen Species

Rabbit

Immunogen

Purified rabbit skeletal α-actinin.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_385929

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actinin alpha 3

Database

Research Area

DATA IMAGES

Anti-sarcomeric alpha Actinin antibody [EA-53] used in IHC (Paraffin sections) (IHC-P). GTX29465

GTX29465 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX29465 sarcomeric alpha Actinin antibody [EA-53].

Anti-sarcomeric alpha Actinin antibody [EA-53] used in IHC (Paraffin sections) (IHC-P). GTX29465

GTX29465 IHC-P Image

The data was published in the journal Cell Death Dis in 2018. PMID: 29371598

REFERENCE

Application Reference

Ma W et al. Nat Commun 2024; 15 (1) : 2137 N-Acetyltransferase 10 represses Uqcr11 and Uqcrb independently of ac4C modification to promote heart regeneration.
Gong R et al. Pharmacol Res 2021; : 105845 Loss of m6A methyltransferase METTL3 promotes heart regeneration and repair after myocardial injury.
Application : IHC-Fr
Reactivity : Mouse
Bellmann J et al. Biomaterials 2019; 225 : 119537 A customizable microfluidic platform for medium-throughput modeling of neuromuscular circuits.
Application : ICC/IF
Reactivity : Human
Williams AL et al. Physiol Genomics 2018; 50 (7) : 479-494 HIF1 mediates a switch in pyruvate kinase isoforms after myocardial infarction.
Milan M et al. Cell Death Dis 2018; 9 (2) : 108 Givinostat reduces adverse cardiac remodeling through regulating fibroblasts activation.
Application : ICC/IF
Reactivity : Mouse
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REVIEW

sarcomeric alpha Actinin antibody [EA-53] Cat. No. GTX29465

Rating
( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-sarcomeric alpha Actinin antibody [EA-53] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX29465
Date : Anonymous submitted on 03-Aug-2018
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Mouse ES cell derived cardiomyocytes
Blocking : 5% BSA,1% goat serum, 0.2% gelatin, 0.1% Triton X-100, 0.1% NaN3, 25°C, 1Hr
Fixation : 4%PFA
Permeabilization /Antigen retrieval : 0.1% Triton X-100 / 5% BSA,1% goat serum, 0.2% gelatin, 0.1% Triton X-100, 0.1% NaN3
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : Green: sarcomeric alpha actinin stained by sarcomeric alpha Actinin antibody [EA-53] (GTX29465) diluted at 1:1000. Blue: DAPI
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399