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sarcomeric alpha Actinin antibody [EA-53]

Anti-sarcomeric alpha Actinin antibody [EA-53] used in IHC (Paraffin sections) (IHC-P). GTX29465

Cat. No. GTX29465

Host

Mouse

Clonality

Monoclonal

Clone Name

EA-53

Isotype

IgG1

Application

ICC/IF, IHC-P, IHC-Fr, IHC-Wm

Reactivity

Human, Mouse, Zebrafish
Package
500 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P 1:20‐1:30
IHC-Fr Assay dependent
IHC-Wm Assay dependent

Note :

IHC-P
Recommendations for antigen retrieval : EDTA buffer pH 8.0 at room temperature for 30 mins.

Not tested in other applications.

Product Note

This antibody is specific for α-skeletal muscle actinin and α-cardiac actinin. It stains Z lines and dots in stress fibers of myotubes in skeletal and cardiac muscle but not in non-sarcomeric muscle elements.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

Purified rabbit skeletal α-actinin.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_385929

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actinin alpha 3

Database

Research Area

DATA IMAGES

Anti-sarcomeric alpha Actinin antibody [EA-53] used in IHC (Paraffin sections) (IHC-P). GTX29465

GTX29465 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX29465 sarcomeric alpha Actinin antibody [EA-53].

REFERENCE

REVIEW

sarcomeric alpha Actinin antibody [EA-53] Cat. No. GTX29465

Rating ( Average 5 based on 2 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2018. PMID: 29371598
Date : Anonymous submitted on 03-Aug-2018
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Mouse ES cell derived cardiomyocytes
Blocking : 5% BSA,1% goat serum, 0.2% gelatin, 0.1% Triton X-100, 0.1% NaN3, 25°C, 1Hr
Fixation : 4%PFA
Permeabilization /Antigen retrieval : 0.1% Triton X-100 / 5% BSA,1% goat serum, 0.2% gelatin, 0.1% Triton X-100, 0.1% NaN3
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : Green: sarcomeric alpha actinin stained by sarcomeric alpha Actinin antibody [EA-53] (GTX29465) diluted at 1:1000. Blue: DAPI
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399