GeneTex
United States (US)

sarcomeric alpha Actinin antibody [EA-53]

GTX29465_20160811_IHC-P_18121410_664.jpg
GTX29465_IHC-P_20181213_1_19011519_700.jpg

Cat No. GTX29465

Host Mouse
Clonality Monoclonal
Clone Name EA-53
Isotype IgG1
Application ICC/IF, IHC-P, IHC-Fr, IHC-Wm
Reactivity Human, Mouse, Rat, Zebrafish, Rabbit, Goat, Sheep, Bovine, Cat, Dog, Hamster, Pig, Xenopus laevis, Lizard, Snake, Fish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
IHC-Wm Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

Diluted ascites containing 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Antigen Species

Rabbit

Immunogen

Purified rabbit skeletal alpha-actinin.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC181036, MGC75587, act, actg, beta-actin, dfna20, dfna26, beta actin, betaactin

Background

This antibody is specific for alpha-skeletal muscle actinin and alpha-cardiac actinin. It stains Z lines and dots in stress fibers of myotubes in skeletal and cardiac muscle but not in non-sarcomeric muscle elements.

Research Area

DATA IMAGES
GTX29465_20160811_IHC-P_18121410_664.jpg

GTX29465 IHC-P Image

Formalin fixed paraffin embedded human skeletal muscle stained with α-Actinin (sarcomeric) antibody.

GTX29465_IHC-P_20181213_1_19011519_700.jpg

GTX29465 IHC-P Image

IHC-P analysis of human skeletal muscle using GTX29465 sarcomeric alpha Actinin antibody [EA-53].

REFERENCE
REVIEW

sarcomeric alpha Actinin antibody [EA-53] Cat No. GTX29465

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 03-Aug-2018
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Mouse ES cell derived cardiomyocytes
Blocking : 5% BSA,1% goat serum, 0.2% gelatin, 0.1% Triton X-100, 0.1% NaN3, 25°C, 1Hr
Fixation : 4%PFA
Permeabilization /Antigen retrieval : 0.1% Triton X-100 / 5% BSA,1% goat serum, 0.2% gelatin, 0.1% Triton X-100, 0.1% NaN3
Primary Antibody : 1:1000, 4°C, overnightHr
Figure Note : Green: sarcomeric alpha actinin stained by sarcomeric alpha Actinin antibody [EA-53] (GTX29465) diluted at 1:1000. Blue: DAPI
Package List Price ($)
$ 319