GeneTex
United States (US)

tPA antibody

GTX103453_40401_IHC_19011506_347.jpg
GTX103453_40401_WB_19011506_566.jpg
GTX103453_40401_WB_R_19011506_365.jpg
GTX103453_40401_IHC_2_19011506_569.jpg
GTX103453_42732_20170223_WB_H_plasma_19011506_315.jpg
GTX103453_40401_WB_M_19011506_312.jpg

Cat No. GTX103453

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

293T , Neuro2A , PC-12 , human plasma

Predict Reactivity

Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human tPA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Plasminogen Activator, Tissue Type,T-Pa,Tpa,Plat

Cellular Localization

Secreted , extracellular space

Background

This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103453_40401_IHC_19011506_347.jpg

GTX103453 IHC-P Image

tPA antibody detects tPA protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
tPA antibody (GTX103453) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103453_40401_WB_19011506_566.jpg

GTX103453 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX103453 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103453_40401_WB_R_19011506_365.jpg

GTX103453 WB Image

tPA antibody detects PLAT protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
tPA antibody (GTX103453) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103453_40401_IHC_2_19011506_569.jpg

GTX103453 IHC-P Image

tPA antibody detects PLAT protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
tPA antibody (GTX103453) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103453_42732_20170223_WB_H_plasma_19011506_315.jpg

GTX103453 WB Image

Human plasma (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with tPA antibody (GTX103453) diluted at 1:2000.

GTX103453_40401_WB_M_19011506_312.jpg

GTX103453 WB Image

tPA antibody detects PLAT protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
7.5% SDS-PAGE
tPA antibody (GTX103453) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf