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tPA antibody

Anti-tPA antibody used in Western Blot (WB). GTX103453
Anti-tPA antibody used in Western Blot (WB). GTX103453
Anti-tPA antibody used in IHC (Paraffin sections) (IHC-P). GTX103453
Anti-tPA antibody used in IHC (Paraffin sections) (IHC-P). GTX103453
Anti-tPA antibody used in Western Blot (WB). GTX103453
Anti-tPA antibody used in Western Blot (WB). GTX103453
Anti-tPA antibody used in Western Blot (WB). GTX103453

Cat. No. GTX103453

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all tPA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

293T , Neuro2A , PC-12 , human plasma

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human tPA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729645

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

plasminogen activator, tissue type , T-PA , TPA

Cellular Localization

Secreted , extracellular space

Background

This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-tPA antibody used in Western Blot (WB). GTX103453

GTX103453 WB Image

tPA antibody detects PLAT protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
tPA antibody (GTX103453) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-tPA antibody used in Western Blot (WB). GTX103453

GTX103453 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX103453 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-tPA antibody used in IHC (Paraffin sections) (IHC-P). GTX103453

GTX103453 IHC-P Image

tPA antibody detects tPA protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
tPA antibody (GTX103453) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-tPA antibody used in IHC (Paraffin sections) (IHC-P). GTX103453

GTX103453 IHC-P Image

tPA antibody detects PLAT protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
tPA antibody (GTX103453) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-tPA antibody used in Western Blot (WB). GTX103453

GTX103453 WB Image

tPA antibody detects PLAT protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
7.5% SDS-PAGE
tPA antibody (GTX103453) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-tPA antibody used in Western Blot (WB). GTX103453

GTX103453 WB Image

Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with tPA antibody (GTX103453) diluted at 1:2000.

Anti-tPA antibody used in Western Blot (WB). GTX103453

GTX103453 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with tPA antibody (GTX103453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Svalina MN et al. Sci Rep 2016; 6 : 27012 IGF1R as a Key Target in High Risk, Metastatic Medulloblastoma.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169