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uPAR antibody [HL2169]

Recombinant antibody KO/KD Orthogonal
Anti-uPAR antibody [HL2169] used in Western Blot (WB). GTX638173
Anti-uPAR antibody [HL2169] used in Western Blot (WB). GTX638173
Anti-uPAR antibody [HL2169] used in IHC (Paraffin sections) (IHC-P). GTX638173
Anti-uPAR antibody [HL2169] used in IHC (Paraffin sections) (IHC-P). GTX638173
Anti-uPAR antibody [HL2169] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638173

Cat. No. GTX638173

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2169

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all uPAR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human uPAR.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

plasminogen activator, urokinase receptor , CD87 , U-PAR , UPAR , URKR

Cellular Localization

Cell membrane,Secreted

Background

This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-uPAR antibody [HL2169] used in Western Blot (WB). GTX638173

GTX638173 WB Image

KO/KD

Wild-type (WT) and PLAUR knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with uPAR antibody [HL2169] (GTX638173) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-uPAR antibody [HL2169] used in Western Blot (WB). GTX638173

GTX638173 WB Image

Orthogonal

Untreated (–) and treated (+) U937 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with uPAR antibody [HL2169] (GTX638173) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-uPAR antibody [HL2169] used in IHC (Paraffin sections) (IHC-P). GTX638173

GTX638173 IHC-P Image

uPAR antibody [HL2169] detects uPAR protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
uPAR stained by uPAR antibody [HL2169] (GTX638173) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-uPAR antibody [HL2169] used in IHC (Paraffin sections) (IHC-P). GTX638173

GTX638173 IHC-P Image

uPAR antibody [HL2169] detects uPAR protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
uPAR stained by uPAR antibody [HL2169] (GTX638173) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-uPAR antibody [HL2169] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638173

GTX638173 ICC/IF Image

uPAR antibody [HL2169] detects uPAR protein at cell membrane and cytoplasmic vesicles by immunofluorescent analysis.
Sample: U937 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: uPAR stained by uPAR antibody [HL2169] (GTX638173) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations