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uPAR antibody

KO/KD Comparison
Anti-uPAR antibody used in Western Blot (WB). GTX100467
Anti-uPAR antibody used in Western Blot (WB). GTX100467
Anti-uPAR antibody used in Western Blot (WB). GTX100467
Anti-uPAR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100467
Anti-uPAR antibody used in IHC (Paraffin sections) (IHC-P). GTX100467
Anti-uPAR antibody used in IHC (Paraffin sections) (IHC-P). GTX100467

Cat. No. GTX100467

Reference 9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all uPAR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

U87-MG , HeLa

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human uPAR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175804

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

plasminogen activator, urokinase receptor , CD87 , U-PAR , UPAR , URKR

Cellular Localization

Isoform 1: Cell membrane; Lipid-anchor , GPI-anchor , Isoform 2: Secreted

Background

This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-uPAR antibody used in Western Blot (WB). GTX100467

GTX100467 WB Image

KO/KD

Wild-type (WT) and uPAR knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with uPAR antibody (GTX100467) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-uPAR antibody used in Western Blot (WB). GTX100467

GTX100467 WB Image

Comparison

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with uPAR antibody (GTX100467) diluted at 1:500 and competitor's antibody (CST#12713) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-uPAR antibody used in Western Blot (WB). GTX100467

GTX100467 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with uPAR antibody (GTX100467) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-uPAR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100467

GTX100467 ICC/IF Image

uPAR antibody detects uPAR protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: uPAR protein stained by uPAR antibody (GTX100467) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-uPAR antibody used in IHC (Paraffin sections) (IHC-P). GTX100467

GTX100467 IHC-P Image

uPAR antibody detects uPAR protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
uPAR stained by uPAR antibody (GTX100467) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-uPAR antibody used in IHC (Paraffin sections) (IHC-P). GTX100467

GTX100467 IHC-P Image

uPAR antibody detects uPAR protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
uPAR stained by uPAR antibody (GTX100467) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

TSR Jensen et al. Inflammation 2024; Urokinase Plasminogen Activator Receptor: An Important Focal Player in Chronic Subdural Hematoma?
Application : IHC-P
Lawaetz M et al. Diagnostics (Basel) 2023; 13 (21) Diagnostic Value of Preoperative uPAR-PET/CT in Regional Lymph Node Staging of Oral and Oropharyngeal Squamous Cell Carcinoma: A Prospective Phase II Trial.
Cardile A et al. Antioxidants (Basel) 2023; 12 (7) Hyperforin Enhances Heme Oxygenase-1 Expression Triggering Lipid Peroxidation in BRAF-Mutated Melanoma Cells and Hampers the Expression of Pro-Metastatic Markers.
Lawaetz M et al. Int J Mol Sci 2023; 24 (4) Potential of uPAR, αvβ6 Integrin, and Tissue Factor as Targets for Molecular Imaging of Oral Squamous Cell Carcinoma: Evaluation of Nine Targets in Primary Tumors and Metastases by Immunohistochemistry.
Application : IHC-P
Reactivity : Human
Elisa De Tomi et al. Int J Mol Sci 2022; 23 (3) Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
TSR Jensen et al. Inflammation 2024; Urokinase Plasminogen Activator Receptor: An Important Focal Player in Chronic Subdural Hematoma?
Application : IHC-P
Lawaetz M et al. Diagnostics (Basel) 2023; 13 (21) Diagnostic Value of Preoperative uPAR-PET/CT in Regional Lymph Node Staging of Oral and Oropharyngeal Squamous Cell Carcinoma: A Prospective Phase II Trial.
Cardile A et al. Antioxidants (Basel) 2023; 12 (7) Hyperforin Enhances Heme Oxygenase-1 Expression Triggering Lipid Peroxidation in BRAF-Mutated Melanoma Cells and Hampers the Expression of Pro-Metastatic Markers.
Lawaetz M et al. Int J Mol Sci 2023; 24 (4) Potential of uPAR, αvβ6 Integrin, and Tissue Factor as Targets for Molecular Imaging of Oral Squamous Cell Carcinoma: Evaluation of Nine Targets in Primary Tumors and Metastases by Immunohistochemistry.
Application : IHC-P
Reactivity : Human
Elisa De Tomi et al. Int J Mol Sci 2022; 23 (3) Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
Application : WB
Reactivity : Human
Chang MC et al. Int Endod J 2020; (Epub) Effects of transforming growth factor-β1 on plasminogen activation in stem cells from the apical papilla: role of activating receptor-like kinase 5/Smad2 and mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) signalling.
Application : WB
Reactivity : Human
Ye JC et al. Journal of Cancer 2017; 8 (15):3028-3036 MZF-1/Elk-1/PKCα is Associated with Poor Prognosis in Patients with Hepatocellular Carcinoma.
Application : WB
Reactivity : Human
Chang MC et al. J Tissue Eng Regen Med 2016; (Epub) Effects of TGF-β1 on plasminogen activation in human dental pulp cells: Role of ALK5/Smad2, TAK1 and MEK/ERK signaling.
Application : WB
Reactivity : Human
Tsai YL et al. J Biol Chem 2015; 290 (13):8049-64 Characterization and mechanism of stress-induced translocation of 78-kilodalton glucose-regulated protein (GRP78) to the cell surface.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169