GeneTex
United States (US)

uPAR antibody

KO/KD Comparison
GTX100467_42501_20170406_WB_KO_watermark_19011506_482.jpg
GTX100467_42501_20171020_WB_competitor_watermark_19011506_818.jpg
GTX100467_40583_IFA_19011506_218.jpg
GTX100467_42816_20171003_IHC-P_19011506_996.jpg
GTX100467_42816_20170420_WB_19011506_594.jpg

Cat No. GTX100467

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

HeLa , U87-MG

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human uPAR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD87 antibody, U-PAR antibody, UPAR antibody, URKR antibody, PLAUR antibody, urokinase plasminogen activator surface receptor antibody, u-plasminogen activator receptor form 2 antibody, monocyte activation antigen Mo3 antibody, plasminogen activator, urokinase receptor antibody

Cellular Localization

Isoform 1: Cell membrane; Lipid-anchor , GPI-anchor , Isoform 2: Secreted

Background

This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100467_42501_20170406_WB_KO_watermark_19011506_482.jpg

GTX100467 WB Image

KO/KD

Wild-type (WT) and uPAR knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with uPAR antibody (GTX100467) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by www.edigene.com

GTX100467_42501_20171020_WB_competitor_watermark_19011506_818.jpg

GTX100467 WB Image

Comparison

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membranes were blotted with uPAR antibody (GTX100467) diluted at 1:500 and competitor's antibody (CST#12713) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX100467_40583_IFA_19011506_218.jpg

GTX100467 ICC/IF Image

uPAR antibody detects uPAR protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: uPAR protein stained by uPAR antibody (GTX100467) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX100467_42816_20171003_IHC-P_19011506_996.jpg

GTX100467 IHC-P Image

uPAR antibody detects uPAR protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
uPAR stained by uPAR antibody (GTX100467) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100467_42816_20170420_WB_19011506_594.jpg

GTX100467 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with uPAR antibody (GTX100467) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf