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xCT / SLC7A11 antibody, Internal

Anti-xCT / SLC7A11 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89082
Anti-xCT / SLC7A11 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89082
Anti-xCT / SLC7A11 antibody, Internal used in Western Blot (WB). GTX89082

Cat. No. GTX89082

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μg ($399)
See all xCT / SLC7A11 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.02-0.06μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89082-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KGQTQNFKDAFSGRD, from the internal region of the protein sequence according to NP_055146.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10721569

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 7 member 11 , CCBR1 , xCT

Cellular Localization

Membrane

Background

This gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. This protein has been identified as the predominant mediator of Kaposi sarcoma-associated herpesvirus fusion and entry permissiveness into cells. Also, increased expression of this gene in primary gliomas (compared to normal brain tissue) was associated with increased glutamate secretion via the XCT channels, resulting in neuronal cell death. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-xCT / SLC7A11 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89082

GTX89082 IHC-P Image

IHC-P analysis of human kidney using GTX89082 SLC7A11 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

Anti-xCT / SLC7A11 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89082

GTX89082 IHC-P Image

IHC-P analysis of human placenta using GTX89082 SLC7A11 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

Anti-xCT / SLC7A11 antibody, Internal used in Western Blot (WB). GTX89082

GTX89082 WB Image

WB analysis of human spleen lysate using GTX89082 SLC7A11 antibody, Internal.
Dilution : 0.02μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

Application Reference

Polewski MD et al. Mol Cancer Res 2016; 14 (12) : 1229-1242 Increased Expression of System xc- in Glioblastoma Confers an Altered Metabolic State and Temozolomide Resistance.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399