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ATP5A1 antibody [C2C3], C-term

Orthogonal Overexpression
Anti-ATP5A1 antibody [C2C3], C-term used in Western Blot (WB). GTX101741
Anti-ATP5A1 antibody [C2C3], C-term used in Western Blot (WB). GTX101741
Anti-ATP5A1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101741
Anti-ATP5A1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101741
Anti-ATP5A1 antibody [C2C3], C-term used in Western Blot (WB). GTX101741
Anti-ATP5A1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101741
Anti-ATP5A1 antibody [C2C3], C-term used in Western Blot (WB). GTX101741
Anti-ATP5A1 antibody [C2C3], C-term used in Western Blot (WB). GTX101741
Anti-ATP5A1 antibody [C2C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX101741
Anti-ATP5A1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101741

Cat. No. GTX101741

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ATP5A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

K562 , THP-1 , mouse brain , rat brain , A431 , HeLa , HepG2 , unboiled ATP5A1-transfected 293T

Predict Reactivity

Bovine, Dog, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ATP5A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618791

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP synthase F1 subunit alpha , ATP5A , ATP5A1 , ATP5AL2 , ATPM , COXPD22 , HEL-S-123m , MC5DN4 , MOM2 , OMR , ORM , hATP1

Cellular Localization

Mitochondrion inner membrane

Background

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the same protein have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq]

Database

  • Gene ID: 498 ATP5F1A
  • UniProt: P25705 ATP5F1A

Research Area

DATA IMAGES

Anti-ATP5A1 antibody [C2C3], C-term used in Western Blot (WB). GTX101741

GTX101741 WB Image

Orthogonal

HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term (GTX101741) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP5A1 antibody [C2C3], C-term used in Western Blot (WB). GTX101741

GTX101741 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody (GTX101741) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP5A1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101741

GTX101741 ICC/IF Image

ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATP5A1 protein stained by ATP5A1 antibody [C2C3], C-term (GTX101741) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-ATP5A1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101741

GTX101741 IHC-P Image

ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
ATP5A1 antibody [C2C3], C-term (GTX101741) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ATP5A1 antibody [C2C3], C-term used in Western Blot (WB). GTX101741

GTX101741 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term (GTX101741) diluted at 1:1000.

Anti-ATP5A1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101741

GTX101741 IHC-P Image

ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at cytoplasm on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
ATP5A1 antibody [C2C3], C-term (GTX101741) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ATP5A1 antibody [C2C3], C-term used in Western Blot (WB). GTX101741

GTX101741 WB Image

ATP5A1 antibody detects ATP5A1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody (GTX101741) diluted by 1:2000.

Anti-ATP5A1 antibody [C2C3], C-term used in Western Blot (WB). GTX101741

GTX101741 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term (GTX101741) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP5A1 antibody [C2C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX101741

GTX101741 IHC-Fr Image

ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: ATP5A1 protein stained by ATP5A1 antibody [C2C3], C-term (GTX101741) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-ATP5A1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101741

GTX101741 IHC-P Image

ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at membrane on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
ATP5A1 antibody [C2C3], C-term (GTX101741) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Mingming Zhao et al. Nat Commun 2022; 13 (1) : 1757 Gut microbiota production of trimethyl-5-aminovaleric acid reduces fatty acid oxidation and accelerates cardiac hypertrophy.
Application : WB
Reactivity : Mouse
Tatsuo Suzuki et al. Life Sci Alliance 2021; 4 (7) Non-microtubule tubulin-based backbone and subordinate components of postsynaptic density lattices.
Application : WB
Lee SO et al. Mol Cancer Res 2014; 12 (4) : 527-538 The orphan nuclear receptor NR4A1 (Nur77) regulates oxidative and endoplasmic reticulum stress in pancreatic cancer cells.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169