Summary
HIF2 alpha antibody recognizes HIF2 alpha / EPAS1 protein, a transcription factor (predicted molecular weight of 97 kDa) induced by hypoxic conditions. It forms a heterodimer with HIF1 beta / ARNT to drive a specific gene expression profile in various physiological and pathological processes that occur in the context of hypoxia.
Application Note
Recommended Starting Dilutions:
For WB: Use at a dilution of 1:100-1:3000. Recognizes a band at 118kDa.
For IHC: Use at a dilution of 1:100.
For ICC/IF: Use at a dilution of 1:100-1:1000.
Optimal dilutions/concentrations should be determined by the researcher.
Calculated MW
Positive Control
HepG2 (1% O2 treatment for 24hr and using Urea buffer) , Myc-DDK human HIF2A-transfected 293T
Product Note
Specific for HIF-2 alpha/EPAS. Does not cross react with HIF-1 alpha.
Predict Reactivity
Human (100%)| Mouse (100%)| Rat (86%)| Bovine (80%)| Chicken (80%)| Pig (93%)
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
A peptide derived from the mouse/human HIF2 alpha.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_370290
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
endothelial PAS domain protein 1 , HIF-2alpha , HIF2A , HLF , HRF , MOP2 , bHLHe73
Background
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells. (1)
Database
Research Area