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BRCA1 antibody [6B4] - ChIP grade

KO/KD
Anti-BRCA1 antibody [6B4] - ChIP grade used in Immunoprecipitation (IP). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in ChIP assay (ChIP assay). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Immunohistochemistry (IHC). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70115
Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115

Cat. No. GTX70115

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6B4

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay, IHC

Reactivity

 Human
Package
100 μl ($399)
See all BRCA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP Assay dependent
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

208 kDa. ( Note )

Positive Control

293T , MCF7 , U2OS , HeLa

Product Note

This antibody recognizes BRCA1, a 220-kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer.

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_368616

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BRCA1 DNA repair associated , BRCAI , BRCC1 , BROVCA1 , FANCS , IRIS , PNCA4 , PPP1R53 , PSCP , RNF53

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]

Database

Research Area

DATA IMAGES

Anti-BRCA1 antibody [6B4] - ChIP grade used in Immunoprecipitation (IP). GTX70115

GTX70115 IP Image

BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) was used for IP-WB assay.
6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract.
Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution.

Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115

GTX70115 WB Image

KO/KD

Wild-type (WT) and BRCA1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [6B4] - ChIP grade (GTX70115) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-BRCA1 antibody [6B4] - ChIP grade used in ChIP assay (ChIP assay). GTX70115

GTX70115 ChIP assay Image

BRCA1 antibody 6B4 (GTX70115) and BRCA1 antibody 17F8 (GTX70111) were used for ChIP assay.
The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 microgram each) in the ChIP assay.
Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.

Anti-BRCA1 antibody [6B4] - ChIP grade used in Immunohistochemistry (IHC). GTX70115

GTX70115 IHC Image

Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115

GTX70115 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [6B4] - ChIP grade (GTX70115) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115

GTX70115 WB Image

BRCA1 antibody detects BRCA1 protein by western blot analysis. Whole cell extracts (30 and 50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody (GTX70115) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115

GTX70115 WB Image

KO/KD

BRCA1 antibody [6B4] (GTX70115) was used at 1:1000 dilution for western blot assay of lysates from cells transfected with control or BRCA1-specific siRNA. Lysates were prepared at the indicated times following transfection. RAD50 antibody [13B3] (GTX70228) was used as a loading control.

Anti-BRCA1 antibody [6B4] - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70115

GTX70115 ICC/IF Image

BRCA1 antibody 6B4 (GTX70115) was used for immunofluorescent staining of BRCA1 nuclear foci induced by ionizing radiation.
IR-treated (2 hr /4 gray IR) U2OS cells were pre-extracted with CSK buffer on ice for 4 min before fixation with 4% PFA in room temperature, and then subjected to immunostaining.
DAPI was used to counterstain nucleus.
6B4 was used at 1:400 dilution.
Secondary antibody (Alexa Fluor-488) used for detection of primary antibody (BRCA1 antibody 6B4).

Anti-BRCA1 antibody [6B4] - ChIP grade used in Western Blot (WB). GTX70115

GTX70115 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [6B4] - ChIP grade (GTX70115) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

REFERENCE

Application Reference

Lajud SA et al. Clin Cancer Res 2014; Dual Disruption of DNA Repair and Telomere Maintenance for the Treatment of Head and Neck Cancer.
Application : WB
Reactivity : Human
Bernard-Gallon DJ et al. Anticancer Res 2004; 24 (1) : 321-4 Differential expressions of BRCA1 and BRCA2 in infantile gynecomastia.
Application : IHC-P
Reactivity : Human
Li S et al. Nature 2000; 406 (6792) : 210-5 Functional link of BRCA1 and ataxia telangiectasia gene product in DNA damage response.
Application : WB, IP
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations