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Paxillin (phospho Tyr118) antibody

Orthogonal
Anti-Paxillin (phospho Tyr118) antibody used in Western Blot (WB). GTX24833
Anti-Paxillin (phospho Tyr118) antibody used in Western Blot (WB). GTX24833

Cat. No. GTX24833

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, IHC

Reactivity

 Human, Mouse, Rat, Pig, Monkey, Xenopus
Package
50 μl ($399)
See all Paxillin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human paxillin that contains tyrosine 118.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380443

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paxillin

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-Paxillin (phospho Tyr118) antibody used in Western Blot (WB). GTX24833

GTX24833 WB Image

Orthogonal

WB analysis of whole cell extracts (30 μg lysate) of COS-7 (Lane 1) treated with 100 uM Pervanadate for 1 hour (Lane 2), A431 (Lane 3) treated with 100 uM Pervanadate for 1 hour (Lane 4), HeLa (Lane 5) treated with 100 uM Pervanadate for 1 hour (Lane 6) using GTX24833 Paxillin (phospho Tyr118) antibody.
Dilution : 1:500

Anti-Paxillin (phospho Tyr118) antibody used in Western Blot (WB). GTX24833

GTX24833 WB Image

Orthogonal

WB analysis of samples using GTX24833 Paxillin (phospho Tyr118) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 6) eliminates the signal further verifying that the antibody is phospho-specific.

REFERENCE

Application Reference

Paron I et al. PLoS One 2011; 6 (6) : e21684 Tenascin-C enhances pancreatic cancer cell growth and motility and affects cell adhesion through activation of the integrin pathway.
Application : WB
Armstrong T et al. Clin Cancer Res 2004; 10 (21) : 7427-37 Type I collagen promotes the malignant phenotype of pancreatic ductal adenocarcinoma.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399