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FLIP antibody

Anti-FLIP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26144
Anti-FLIP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26144
Anti-FLIP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26144
Anti-FLIP antibody used in Western Blot (WB). GTX26144

Cat. No. GTX26144

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($479)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1μg/ml
ICC/IF 10μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to aa 447- 464 at the C-terminus of human FLIPα/FLIPL (accession no. AAC51622)

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_368660

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CASH , CASP8 and FADD like apoptosis regulator , CASP8AP1 , CFLAR , CLARP , Casper , FLAME , FLAME1 , FLIP , IFLICE , MRIT , cFLIP , cFLIPL , cFLIPR , cFLIPS

Background

The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-FLIP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26144

GTX26144 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX26144 FLIP antibody.
Dilution : 10 μg/ml

Anti-FLIP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26144

GTX26144 ICC/IF Image

ICC/IF analysis of K562 cells using GTX26144 FLIP antibody.
Red : Primary antibody
Blue : DAPI
Dilution : 5 μg/ml

Anti-FLIP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26144

GTX26144 ICC/IF Image

Immunocytochemical staining of FLIPα in Jurkat cells with FLIPα antibody (GTX26144) at 10μg/ml.

Anti-FLIP antibody used in Western Blot (WB). GTX26144

GTX26144 WB Image

Western blot analysis of FLIP in HeLa (H), Jurkat (J), and K562 (K) whole cell lysate with FLIP antibody (GTX26144) at 1ug/ml. Positive control: Whole cell lysate from HeLa cells.

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 479