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DDB1 antibody

Anti-DDB1 antibody used in Western Blot (WB). GTX70307
Anti-DDB1 antibody used in Western Blot (WB). GTX70307

Cat. No. GTX70307

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all DDB1 products

APPLICATION

Application Note

For WB: Use at an assay dependent dilution. Predicted molecular weight: 127 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

127 kDa. ( Note )

Positive Control

T47D and MCF-7 whole cell lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide conugated to protein carrier.

Purification

Affinity purified with antigen
From polyclonal serum

Conjugation

Unconjugated

RRID

AB_369932

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

damage specific DNA binding protein 1 , DDBA , UV-DDB1 , XAP1 , XPCE , XPE , XPE-BF

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins. [provided by RefSeq, May 2012]

Database

Research Area

DATA IMAGES

Anti-DDB1 antibody used in Western Blot (WB). GTX70307

GTX70307 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody (GTX70307) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-DDB1 antibody used in Western Blot (WB). GTX70307

GTX70307 WB Image

Detection of Human DDB1 in (1) Jurkat, (2) MCF7, and (3) T47D cell lysates using GeneTex polyclonal antibody GTX70307

REFERENCE

Application Reference

Sansam CL et al. Genes Dev 2006; 20 (22) : 3117-29 DTL/CDT2 is essential for both CDT1 regulation and the early G2/M checkpoint.
Application : WB
Reactivity : Human
Submit a Reference

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Package List Price ($)
$ 399