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Hec1 antibody [9G3.23]

Orthogonal
Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268
Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268
Anti-Hec1 antibody [9G3.23] used in IHC (Frozen sections) (IHC-Fr). GTX70268
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

Cat. No. GTX70268

Reference 85
2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9G3.23

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-Fr, FACS, IP, PLA

Reactivity

 Human, Mouse, Hamster, Kangaroo rat
Package
100 μl ($399)
See all Hec1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

293T , A431 , HepG2 , HeLa, U87-MG NE nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human HEC1 protein consisting of amino acids 56-642.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_371632

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80

Cellular Localization

Nucleus

Background

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268

GTX70268 WB Image

HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] (GTX70268) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate (GTX14698).

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

Orthogonal

Hec1 antibody [9G3.23] detects Hec1 protein at kinetochore by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Hec1 stained by Hec1 antibody [9G3.23] (GTX70268) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (GTX102079) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

Hec1 antibody [9G3.23] detects Hec1 protein at kinetochore by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Hec1 stained by Hec1 antibody [9G3.23] (GTX70268) diluted at 1:500.
Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody (GTX112141) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268

GTX70268 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hec1 antibody [9G3.23] (GTX70268) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

Mouse anti-Hec1 (9G3, cat# GTX 70268) and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.

Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268

GTX70268 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hec1 antibody [9G3.23] (GTX70268) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

Hec1 antibody [9G3.23] detects Hec1 protein at kinetochore by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Hec1 stained by Hec1 antibody [9G3.23] (GTX70268) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

Hec1 antibody [9G3.23] detects Hec1 protein at kinetochore by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Hec1 stained by Hec1 antibody [9G3.23] (GTX70268) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268

GTX70268 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2016.PMID: 27764815

Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268

GTX70268 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2017.PMID: 29100415

Anti-Hec1 antibody [9G3.23] used in IHC (Frozen sections) (IHC-Fr). GTX70268

GTX70268 IHC-Fr Image

The data was published in the journal PLoS One in 2010.PMID: 21187932

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

The data was published in the journal Nat Commun in 2015.PMID: 26620470

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

The data was published in the journal Biol Open in 2015.PMID: 26685311

Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268

GTX70268 WB Image

The data was published in the journal Biol Open in 2015.PMID: 26685311

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

The data was published in the journal Nat Commun in 2020. PMID: 32001712

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

The data was published in the journal PLoS One in 2014. PMID: 25329824

Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268

GTX70268 ICC/IF Image

The data was published in the journal Nat Commun in 2020. PMID: 32001712

REFERENCE

Application Reference

Mason FM et al. Proc Natl Acad Sci U S A 2023; 120 (39) : e2303752120 SETD2 safeguards the genome against isochromosome formation.
Rahi A et al. J Cell Biol 2023; 222 (8) The Ndc80-Cdt1-Ska1 complex is a central processive kinetochore-microtubule coupling unit.
Schrock MS et al. J Cell Sci 2022; 135 (12) MKLP2 functions in early mitosis to ensure proper chromosome congression.
Zadra I et al. Nat Commun 2022; 13 (1) : 7147 Chromosome segregation fidelity requires microtubule polyglutamylation by the cancer downregulated enzyme TTLL11.
Jihee Hong et al. J Ginseng Res 2022; 46 (3) : 481-488 Ginsenoside Rg1 suppresses cancer cell proliferation through perturbing mitotic progression.
Application : ICC/IF
Show More +
Submit a Reference

REVIEW

Hec1 antibody [9G3.23] Cat. No. GTX70268

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-Hec1 antibody [9G3.23] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70268
Date : Anonymous submitted on 31-Oct-2013
Score :
Application Tested : ICC/IF
Sample Species : Hm
Sample : CHO cells
Blocking : 1% BSA in PBS, 37°C, 0.5Hr
Fixation : 4% PFA in Phem buffer, 37°C, 0.3Hr
Permeabilization /Antigen retrieval : 0.5% Triton, 37°C, 5Hr
Primary Antibody : 1:250, 37°C, 1Hr
Figure Note : The image shows a CHO cell in prometaphase stained with Hec1 antibody(GTX70268) (green) and DNA (blue). The scale bar is 5 μM.
Anti-Hec1 antibody [9G3.23] used in Western Blot (WB). GTX70268
Date : Anonymous submitted on 23-Mar-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 50μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 3Hr
Submit a Review
Application Reference
Mason FM et al. Proc Natl Acad Sci U S A 2023; 120 (39):e2303752120 SETD2 safeguards the genome against isochromosome formation.
Rahi A et al. J Cell Biol 2023; 222 (8) The Ndc80-Cdt1-Ska1 complex is a central processive kinetochore-microtubule coupling unit.
Schrock MS et al. J Cell Sci 2022; 135 (12) MKLP2 functions in early mitosis to ensure proper chromosome congression.
Zadra I et al. Nat Commun 2022; 13 (1):7147 Chromosome segregation fidelity requires microtubule polyglutamylation by the cancer downregulated enzyme TTLL11.
Jihee Hong et al. J Ginseng Res 2022; 46 (3):481-488 Ginsenoside Rg1 suppresses cancer cell proliferation through perturbing mitotic progression.
Application : ICC/IF
April L Solon et al. Sci Adv 2021; 7 (47):eabj9812 Kinesin-binding protein remodels the kinesin motor to prevent microtubule binding.
Application : ICC/IF
Reactivity : Human
Steblyanko Y et al. EMBO J 2020; 39 (23):e105432 Microtubule poleward flux in human cells is driven by the coordinated action of four kinesins.
Broad AJ et al. J Cell Biol 2020; 219 (3) Aurora B kinase is recruited to multiple discrete kinetochore and centromere regions in human cells.
Liang C et al. J Cell Biol 2020; 219 (2) Centromere-localized Aurora B kinase is required for the fidelity of chromosome segregation.
Yoshida S et al. Nat Commun 2020; 11 (1):2652 Prc1-rich kinetochores are required for error-free acentrosomal spindle bipolarization during meiosis I in mouse oocytes.
Reactivity : Mouse
Kim S et al. Nat Commun 2020; 11 (1):612 PRMT6-mediated H3R2me2a guides Aurora B to chromosome arms for proper chromosome segregation.
Application : ICC/IF
Reactivity : Human
Chuang SH et al. Eur J Med Chem 2020; 191:112118 Discovery of T-1101 tosylate as a first-in-class clinical candidate for Hec1/Nek2 inhibition in cancer therapy.
Application : WB
Reactivity : Human
Malaby HLH et al. J Cell Biol 2019; 218 (4):1218-1234 Kinesin-binding protein ensures accurate chromosome segregation by buffering KIF18A and KIF15.
Li W et al. Sci Adv 2019; 5 (3):eaau7566 A methylation-phosphorylation switch determines Plk1 kinase activity and function in DNA damage repair.
Gwon D et al. Mol Cells 2019; 42 (12):840-849 c-Cbl Acts as an E3 Ligase Against DDA3 for Spindle Dynamics and Centriole Duplication during Mitosis.
Application : WB
Asai Y et al. J Cell Biol 2019; 218 (10):3223-3236 Aurora B kinase activity is regulated by SET/TAF1 on Sgo2 at the inner centromere.
Application : WB
Reactivity : Human
Kim S et al. Toxicol In Vitro 2019; 59:115-125 Bisphenol A disrupts mitotic progression via disturbing spindle attachment to kinetochore and centriole duplication in cancer cell lines.
Application : ICC/IF
Reactivity : Human
Hayward D et al. J Cell Biol 2019; (Epub) CDK1-CCNB1 creates a spindle checkpoint-permissive state by enabling MPS1 kinetochore localization.
Application : ICC/IF
Reactivity : Human
Heidi LH Malaby et al. Life Sci Alliance 2019; 2 (1):e201800169 KIF18A's neck linker permits navigation of microtubule-bound obstacles within the mitotic spindle.
Application : ICC/IF
Reactivity : Human
Alfonso-P?rez T et al. J Cell Biol 2019; (Epub) MAD1-dependent recruitment of CDK1-CCNB1 to kinetochores promotes spindle checkpoint signaling.
Ferrara M et al. Oncogene 2018; 37 (2):231-240 Small molecules targeted to the microtubule-Hec1 interaction inhibit cancer cell growth through microtubule stabilization.
Nye J et al. PLoS One 2018; 13 (10):e0205948 HJURP antagonizes CENP-A mislocalization driven by the H3.3 chaperones HIRA and DAXX.
Application : WB
Reactivity : Human
Hellmuth S et al. EMBO J 2018; 37 (22) Local activation of mammalian separase in interphase promotes double-strand break repair and prevents oncogenic transformation.
Application : ICC/IF
Reactivity : Human
Janssen LME et al. Curr Biol 2018; (Epub) Loss of Kif18A Results in Spindle Assembly Checkpoint Activation at Microtubule-Attached Kinetochores.
Application : ICC/IF
Reactivity : Human
Petsalaki E et al. Chromosoma 2018; (Epub) Chmp4c is required for stable kinetochore-microtubule attachments.
Application : ICC/IF
Reactivity : Human
Heidi Malaby et al. bioRxiv 2018; (Epub) Kinesin Binding Protein (KBP) buffers the activity of Kif18A and Kif15 in mitosis to ensure accurate chromosome segregation.
Application : ICC/IF
Reactivity : Human
Wolf PG et al. J Cell Sci 2018; (Epub) Studying meiotic cohesin in somatic cells reveals that Rec8-cohesin requires Stag3 and is regulated by Wapl and Sororin.
Application : ICC/IF
Reactivity : Human
Petsalaki E et al. J Cell Biol 2018; (Epub) The ESCRT protein Chmp4c regulates mitotic spindle checkpoint signaling.
Application : ICC/IF
Reactivity : Human
Shirnekhi HK et al. Mol Biol Cell 2017; 28 (15):2035-2041 Spindle assembly checkpoint signaling and sister chromatid cohesion are disrupted by HPV E6-mediated transformation.
Application : ICC/IF
Reactivity : Human
Lee M et al. Oncotarget 2017; 8 (44):77649-77672 The E2F activators control multiple mitotic regulators and maintain genomic integrity through Sgo1 and BubR1.
Application : WB
Reactivity : Human
Park JE et al. Toxicol Lett 2017; 281:110-118 The tobacco carcinogen NNK disturbs mitotic chromosome alignment by interrupting p53 targeting to the centrosome.
Application : ICC/IF
Reactivity : Human
Takagi M et al. bioRxiv 2017;:Epub ahead of print Ki-67 and condensins support the integrity of mitotic chromosomes through distinct mechanisms.
Application : ICC/IF
Reactivity : Human
Niikura Y et al. Cell Cycle 2017; 16 (18):1683-1694 SGT1-HSP90 complex is required for CENP-A deposition at centromeres.
Application : ICC/IF
Reactivity : Human
Janczyk P? et al. Dev Cell 2017; 41 (4):438-449.e4 Mechanism of Ska Recruitment by Ndc80 Complexes to Kinetochores.
Application : ICC/IF, PLA
Reactivity : Human
Brouwers N et al. PLoS One 2017; 12 (4):e0174819 Role of Kif15 and its novel mitotic partner KBP in K-fiber dynamics and chromosome alignment.
Application : ICC/IF
Reactivity : Human
Huis In 't Veld PJ et al. Elife 2016; 5 Molecular basis of outer kinetochore assembly on CENP-T.
Petrovic A et al. Cell 2016; 167 (4):1028-1040.e15 Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores.
Fang Y et al. Oncotarget 2016; 7 (48):79327-79341 Preclinical activity of MBM-5 in gastrointestinal cancer by inhibiting NEK2 kinase activity.
Application : WB
Reactivity : Human
Zhou H et al. Nat Commun 2016; 7:10151 Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2.
Application : ICC/IF
Reactivity : Human
Petrovic A et al. Cell 2016; 167 (4):1028-1040 Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores.
Application : WB
Reactivity : Human
Redli PM et al. J Cell Biol 2016; 215 (1):77-93 The Ska complex promotes Aurora B activity to ensure chromosome biorientation.
Application : ICC/IF
Reactivity : Human
Chung HJ et al. J Biol Chem 2016; (Epub) Phosphorylation of Astrin regulates its Kinetochore Function.
Application : ICC/IF
Reactivity : Human
DeLuca KF et al. Methods Mol Biol 2016; 1413:147-68 Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells.
Application : ICC/IF
Reactivity : Human
Zhu L et al. J Biol Chem 2015; 290 (45):27053-66 Mitotic Protein CSPP1 Interacts with CENP-H Protein to Coordinate Accurate Chromosome Oscillation in Mitosis.
O'Connor A et al. Biol Open 2015; 5 (1):43413 Requirement for PLK1 kinase activity in the maintenance of a robust spindle assembly checkpoint.
Application : ICC/IF
Reactivity : Human
Tauchman EC et al. Nat Commun 2015; 6:10036 Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells.
Kaczmarczyk A et al. PLoS One 2014; 9 (10):e106464 CENP-W plays a role in maintaining bipolar spindle structure.
Application : ICC/IF
Reactivity : Human
Espert A et al. J Cell Biol 2014; 206 (7):833-42 PP2A-B56 opposes Mps1 phosphorylation of Knl1 and thereby promotes spindle assembly checkpoint silencing.
Reactivity : Human
Hafner J et al. Nat Commun 2014; 5:4397 Pre-anaphase chromosome oscillations are regulated by the antagonistic activities of Cdk1 and PP1 on Kif18A.
Application : ICC/IF
Reactivity : Human
Huang LY et al. Cancer Chemother Pharmacol 2014; Inhibition of Hec1 as a novel approach for treatment of primary liver cancer.
Application : WB
Basilico F et al. Elife 2014; 3:e02978 The pseudo GTPase CENP-M drives human kinetochore assembly.
Application : WB, ICC/IF
Reactivity : Human
Matson DR et al. J Cell Biol 2014; 205 (4):541-554 CENP-I and Aurora B act as a molecular switch that ties RZZ/Mad1 recruitment to kinetochore attachment status.
Application : ICC/IF
Reactivity : Human
Eleni Petsalaki et al. Journal of Cell Biology 2014; 205 (3):339-56 Chk2 prevents mitotic exit when the majority of kinetochores are unattached
Application : ICC/IF
Reactivity : Human
Huang LY et al. Mol Cancer Ther 2014; Activity of a Novel HEC1-Targeted Anticancer Compound Against Breast Cancer Cell Lines In Vitro and In Vivo.
Application : WB, ICC/IF
Reactivity : Human
Caldas GV et al. J Cell Biol 2013; 203 (6):957-69 KNL1 facilitates phosphorylation of outer kinetochore proteins by promoting Aurora B kinase activity.
Application : WB
Reactivity : Human
Buheitel J et al. EMBO J 2013; 32 (5):666-76 Prophase pathway-dependent removal of cohesin from human chromosomes requires opening of the Smc3-Scc1 gate.
Application : ICC/IF
Reactivity : Human
Beck J et al. Nat Cell Biol 2013; 15 (4):430-9 Ubiquitylation-dependent localization of PLK1 in mitosis.
Application : ICC/IF
Reactivity : Human
Lawo S et al. Nat Cell Biol 2012; 14 (11):1148-58 Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material.
Application : WB
De Antoni A et al. J Cell Biol 2012; 199 (2):269-84 A small-molecule inhibitor of Haspin alters the kinetochore functions of Aurora B.
Matson DR et al. Genes Dev 2012; 26 (6):542-7 A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint.
Chan YW et al. J Cell Biol 2012; 196 (5):563-71 Aurora B controls kinetochore-microtubule attachments by inhibiting Ska complex-KMN network interaction.
Krenn V et al. J Cell Biol 2012; 196 (4):451-67 Structural analysis reveals features of the spindle checkpoint kinase Bub1-kinetochore subunit Knl1 interaction.
Wei R et al. Mol Biol Cell 2011; 22 (19):3584-94 Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint.
Application : ICC/IF
Reactivity : Kangaroo rat
Serio G et al. Proc Natl Acad Sci U S A 2011; 108 (42):17337-42 Small GTPase Rab5 participates in chromosome congression and regulates localization of the centromere-associated protein CENP-F to kinetochores.
Application : ICC/IF
Reactivity : Human
Screpanti E et al. Curr Biol 2011; 21 (5):391-8 Direct binding of Cenp-C to the Mis12 complex joins the inner and outer kinetochore.
Application : ICC/IF
Reactivity : Human
Tooley JG et al. Mol Biol Cell 2011; 22 (8):1217-26 The Ndc80 complex uses a tripartite attachment point to couple microtubule depolymerization to chromosome movement.
Application : WB
Reactivity : Human
Sundin LJ et al. Mol Biol Cell 2011; 22 (6):759-68 The NDC80 complex proteins Nuf2 and Hec1 make distinct contributions to kinetochore-microtubule attachment in mitosis.
Application : ICC/IF
Reactivity : Human
Barnhart MC et al. J Cell Biol 2011; 194 (2):229-43 HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore.
Application : ICC/IF
Reactivity : Human
Santaguida S et al. EMBO J 2011; 30 (8):1508-19 Evidence that Aurora B is implicated in spindle checkpoint signalling independently of error correction.
Application : ICC/IF
Reactivity : Human
Petrovic A et al. J Cell Biol 2010; 190 (5):835-52 The MIS12 complex is a protein interaction hub for outer kinetochore assembly.
Application : WB
Zanet J et al. PLoS One 2010; 5 (12):e15701 A mitosis block links active cell cycle with human epidermal differentiation and results in endoreplication.
Application : IHC-Fr
Reactivity : Human
Zeng K et al. J Cell Biol 2010; 191 (7):1315-32 Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.
Screpanti E et al. PLoS One 2010; 5 (7):e11603 A screen for kinetochore-microtubule interaction inhibitors identifies novel antitubulin compounds.
Application : ICC/IF
Santaguida S et al. J Cell Biol 2010; 190 (1):73-87 Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine.
Application : ICC/IF
Reactivity : Human
Wu G et al. Mol Biol Cell 2009; 20 (22):4686-95 Hec1 contributes to mitotic centrosomal microtubule growth for proper spindle assembly through interaction with Hice1.
Application : ICC/IF
Reactivity : Human
Chan YW et al. J Cell Biol 2009; 185 (5):859-74 Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly.
Application : ICC/IF
Reactivity : Human
Wu G et al. Cancer Res 2008; 68 (20):2371765 Small molecule targeting the Hec1/Nek2 mitotic pathway suppresses tumor cell growth in culture and in animal.
Application : ICC/IF
Barr AR et al. Mol Cell Biol 2008; 28 (23):7199-211 MCAK-independent functions of ch-Tog/XMAP215 in microtubule plus-end dynamics.
Application : ICC/IF
Reactivity : Human
Zhu H et al. J Cell Biol 2008; 183 (5):835-48 FAM29A promotes microtubule amplification via recruitment of the NEDD1-gamma-tubulin complex to the mitotic spindle.
Application : ICC/IF
Reactivity : Human
Miller SA et al. Curr Biol 2008; 18 (22):1785-91 Kinetochore attachments require an interaction between unstructured tails on microtubules and Ndc80(Hec1).
Application : ICC/IF
Reactivity : Human
Jang CY et al. J Cell Biol 2008; 181 (2):255-67 DDA3 recruits microtubule depolymerase Kif2a to spindle poles and controls spindle dynamics and mitotic chromosome movement.
Application : ICC/IF
Reactivity : Human
Wong J et al. Mol Biol Cell 2008; 19 (5):2083-91 Aurora A regulates the activity of HURP by controlling the accessibility of its microtubule-binding domain.
Application : ICC/IF
Reactivity : Human
Montembault E et al. J Cell Biol 2007; 179 (4):601-9 PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores.
Application : ICC/IF
Reactivity : Human
Wong J et al. J Cell Biol 2006; 173 (6):879-91 HURP controls spindle dynamics to promote proper interkinetochore tension and efficient kinetochore capture.
Application : ICC/IF
Reactivity : Human
Klein UR et al. Mol Biol Cell 2006; 17 (6):2547-58 Centromere targeting of the chromosomal passenger complex requires a ternary subcomplex of Borealin, Survivin, and the N-terminal domain of INCENP.
Application : WB
Reactivity : Human
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