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Actin antibody [mAbGEa]

Anti-Actin antibody [mAbGEa] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80809
Anti-Actin antibody [mAbGEa] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80809
Anti-Actin antibody [mAbGEa] used in IHC (Paraffin sections) (IHC-P). GTX80809
Anti-Actin antibody [mAbGEa] used in IHC (Paraffin sections) (IHC-P). GTX80809
Anti-Actin antibody [mAbGEa] used in IHC (Paraffin sections) (IHC-P). GTX80809
Anti-Actin antibody [mAbGEa] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80809

Cat. No. GTX80809

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 mAbGEa

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP, ELISA, ChIP assay, IHC

Reactivity

 Human, Mouse, Rat, Zebrafish, Yeast, Sheep, Drosophila, Bovine, Pig, Xenopus, Plant, Bacteria
Package
100 μl ($399)
See all Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF 1:10-1:100
IHC-P 1:100
IP Assay dependent
ELISA Assay dependent
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Product Note

This antibody has shown cross-reactivity with actin 1, 2, 3, 4, 7, 8, 11 and 12.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Arabidopsis thaliana

Immunogen

Purified Arabidopsis actin protein.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_626122

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin alpha cardiac muscle 1 , ACTC , ASD5 , CMD1R , CMH11 , LVNC4

Cellular Localization

Cytoplasm, cytoskeleton

Background

Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq, Jul 2008]

Database

  • Gene ID: 70 ACTC1
  • UniProt: P68032 ACTC1

Research Area

DATA IMAGES

Anti-Actin antibody [mAbGEa] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80809

GTX80809 ICC/IF Image

ICC/IF analysis of 3T3 cells using GTX80809 Actin antibody [mAbGEa].
Green : Primary antibody
Blue : cell nucleus
Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature
Dilution : 1:100 in blocking buffer for at least 1 hour at room temperature

Anti-Actin antibody [mAbGEa] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80809

GTX80809 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX80809 Actin antibody [mAbGEa]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-Actin antibody [mAbGEa] used in IHC (Paraffin sections) (IHC-P). GTX80809

GTX80809 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX80809 Actin antibody [mAbGEa].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:1000

Anti-Actin antibody [mAbGEa] used in IHC (Paraffin sections) (IHC-P). GTX80809

GTX80809 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX80809 Actin antibody [mAbGEa].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:1000

Anti-Actin antibody [mAbGEa] used in IHC (Paraffin sections) (IHC-P). GTX80809

GTX80809 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX80809 Actin antibody [mAbGEa].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:1000

Anti-Actin antibody [mAbGEa] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80809

GTX80809 ICC/IF Image

ICC/IF analysis of A375 Cells using GTX80809 Actin antibody [mAbGEa]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

REFERENCE

Application Reference

Zhang T et al. Cell 2020; 180 (6) : 1115-1129 Influenza Virus Z-RNAs Induce ZBP1-Mediated Necroptosis.
Application : WB
Valencia-Cervantes J et al. Oncol Rep 2019; 41 (1) : 178-190 Hypoxia Increases Chemoresistance in Human Medulloblastoma DAOY Cells via Hypoxia?inducible Factor 1α?mediated Downregulation of the CYP2B6, CYP3A4 and CYP3A5 Enzymes and Inhibition of Cell Proliferation.
Kryndushkin DS et al. EMBO J 2008; 27 (20) : 2725-35 Curing of the [URE3] prion by Btn2p, a Batten disease-related protein.
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399