p53 (phospho Ser15) antibody

Cat. No. GTX21431

Reference 7

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP, ELISA, IHC
Reactivity	Human, Mouse, Rat
Package	50 μg ($399)

See all p53 products

View product citations for antibody GTX21431 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-P	Assay dependent
IP	Assay dependent
ELISA	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Product Note

The phospho-p53 (Ser15) antibody detects ~53 kDa phosphorylated p53 at Ser15. It does not react with non-phosphorylated p53 or p53 phosphorylated at other sites.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 30% Glycerol, 0.5% BSA

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic phospho-peptide surrounding amino acid Ser 15 of human p53.

Conjugation

Unconjugated

RRID

AB_367777

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Cellular Localization

Cytoplasm,Nucleus,Endoplasmic reticulum,Mitochondrion matrix

Background

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. p53 can apparently be phosphorylated by ATM, ATR, and DNA-PK at Ser15; the phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage.

Database

Gene ID: 7157 TP53
UniProt: P04637 TP53

Research Area

DATA IMAGES

GTX21431 WB Image

WB analysis of various samples using GTX21431 p53 (phospho Ser15) antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate

GTX21431 IHC-P Image

IHC-P analysis of human breast cancer using GTX21431 p53 (phospho Ser15) antibody.
Aantigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0).

GTX21431 ELISA Image

ELISA analysis of phospho- and non phosho- peptide using GTX21431 p53 (phospho Ser15) antibody.
Red : Phosphopeptide
Blue :Non-phosphopeptide
Dilution : 5 μg/ml