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FEN1 antibody [4E7]

KO/KD
Anti-FEN1 antibody [4E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70185
Anti-FEN1 antibody [4E7] used in Western Blot (WB). GTX70185
Anti-FEN1 antibody [4E7] used in Western Blot (WB). GTX70185
Anti-FEN1 antibody [4E7] used in Western Blot and ChIP assay (WB ChIP assay). GTX70185

Cat. No. GTX70185

Reference 26

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4E7

Isotype

 IgG1

Application

 WB, ICC/IF, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all FEN1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IP Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

MCF7

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human FEN-1 protein encoding amino acids 1-380 purified E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_370522

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

flap structure-specific endonuclease 1 , FEN-1 , MF1 , RAD2

Cellular Localization

nucleoplasm

Background

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-FEN1 antibody [4E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70185

GTX70185 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF7, using FEN1 [4E7](GTX70185) antibody at 1:200 dilution.

Anti-FEN1 antibody [4E7] used in Western Blot (WB). GTX70185

GTX70185 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with FEN1 antibody (GTX70185) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-FEN1 antibody [4E7] used in Western Blot (WB). GTX70185

GTX70185 WB Image

KO/KD

The data was published in the journal PLoS Pathog in 2018.PMID: 29928064

Anti-FEN1 antibody [4E7] used in Western Blot and ChIP assay (WB ChIP assay). GTX70185

GTX70185 WB ChIP assay Image

The data was published in the journal EMBO J in 2018.PMID: 29773570

REFERENCE

Application Reference

Hao Q et al. Sci Immunol 2023; 8 (81) : eade1167 DNA repair mechanisms that promote insertion-deletion events during immunoglobulin gene diversification.
Ma Z et al. Redox Biol 2020; : 101653 Symmetrical dimethylation of H4R3: A bridge linking DNA damage and repair upon oxidative stress.
Application : WB
Reactivity : Human
Li JL et al. Cancer Med 2019; (Epub) FEN1 inhibitor increases sensitivity of radiotherapy in cervical cancer cells.
Application : WB
Reactivity : Human
Kanagaraj A et al. Biochem Biophys Res Commun 2019; Different antiviral activities of natural APOBEC3C, APOBEC3G, and APOBEC3H variants against hepatitis B virus.
Application : WB
Reactivity : Human
Kitamura K et al. PLoS Pathog 2018; 14 (6) : e1007124 Flap endonuclease 1 is involved in cccDNA formation in the hepatitis B virus.
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REVIEW

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Application Reference
Hao Q et al. Sci Immunol 2023; 8 (81):eade1167 DNA repair mechanisms that promote insertion-deletion events during immunoglobulin gene diversification.
Ma Z et al. Redox Biol 2020;:101653 Symmetrical dimethylation of H4R3: A bridge linking DNA damage and repair upon oxidative stress.
Application : WB
Reactivity : Human
Li JL et al. Cancer Med 2019; (Epub) FEN1 inhibitor increases sensitivity of radiotherapy in cervical cancer cells.
Application : WB
Reactivity : Human
Kanagaraj A et al. Biochem Biophys Res Commun 2019; Different antiviral activities of natural APOBEC3C, APOBEC3G, and APOBEC3H variants against hepatitis B virus.
Application : WB
Reactivity : Human
Kitamura K et al. PLoS Pathog 2018; 14 (6):e1007124 Flap endonuclease 1 is involved in cccDNA formation in the hepatitis B virus.
Li Z et al. EMBO J 2018; (Epub) hDNA2 nuclease/helicase promotes centromeric DNA replication and genome stability.
Application : IP
Reactivity : Human
Zhang K et al. Am J Pathol 2017; (Epub) Overexpression of Flap Endonuclease 1 Correlates with Enhanced Proliferation and Poor Prognosis of Non-Small-Cell Lung Cancer.
Application : WB
Reactivity : Human
Briest F et al. Neuroendocrinology 2017; (Epub) Mechanisms of targeting the MDM2-p53-FOXM1 axis in well-differentiated intestinal neuroendocrine tumors.
Application : WB
Reactivity : Human
Tsai YC et al. J Nucleic Acids 2017; 2017:8154646 Overexpression of PCNA Attenuates Oxidative Stress-Caused Delay of Gap-Filling during Repair of UV-Induced DNA Damage.
Application : WB
Reactivity : Human
Sami F et al. Biochem J 2015; 468 (2):227-44 RECQ1 interacts with FEN-1 and promotes binding of FEN-1 to telomeric chromatin.
Application : ICC/IF
Reactivity : Human
EL Baple et al. J Clin Invest 2014; 124 (7):3137-46 Hypomorphic PCNA mutation underlies a human DNA repair disorder
Kazak L et al. PLoS One 2013; 8 (5):e62340 A cryptic targeting signal creates a mitochondrial FEN1 isoform with tailed R-Loop binding properties.
Application : WB
Reactivity : Human
Tann AW et al. J Biol Chem 2011; 286 (37):31975-83 Apoptosis induced by persistent single-strand breaks in mitochondrial genome: critical role of EXOG (5'-EXO/endonuclease) in their repair.
Asagoshi K et al. DNA Repair (Amst) 2010; 9 (2):109-19 DNA polymerase beta-dependent long patch base excision repair in living cells.
Guo Z et al. Nat Chem Biol 2010; 6 (10):766-73 Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding.
Application : IP
Reactivity : Human
Masaoka A et al. DNA Repair (Amst) 2009; 8 (11):1290-9 DNA polymerase beta and PARP activities in base excision repair in living cells.
Kong X et al. Nucleic Acids Res 2009; 37 (9):e68 Comparative analysis of different laser systems to study cellular responses to DNA damage in mammalian cells.
Application : ICC/IF
Reactivity : Human
Guo Z et al. Nucleic Acids Res 2009; 37 (10):3431-41 Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity.
Application : WB
Reactivity : Mouse
Szczesny B et al. J Biol Chem 2008; 283 (39):26349-56 Long patch base excision repair in mammalian mitochondrial genomes.
Liu P et al. Mol Cell Biol 2008; 28 (16):4975-87 Removal of oxidative DNA damage via FEN1-dependent long-patch base excision repair in human cell mitochondria.
Reactivity : Human
Guo Z et al. Mol Cell Biol 2008; 28 (13):880479 Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair.
Application : WB
Reactivity : Human
Guo Z et al. J Mol Biol 2008; 377 (3):679-90 Comprehensive mapping of the C-terminus of flap endonuclease-1 reveals distinct interaction sites for five proteins that represent different DNA replication and repair pathways.
Application : WB
Prasad R et al. Mol Cell 2007; 27 (5):829-41 HMGB1 is a cofactor in mammalian base excision repair.
Application : WB
Reactivity : Mouse
Heale JT et al. Mol Cell 2006; 21 (6):837-48 Condensin I interacts with the PARP-1-XRCC1 complex and functions in DNA single-strand break repair.
Kedar PS et al. J Biol Chem 2002; 277 (34):31115-23 Direct interaction between mammalian DNA polymerase beta and proliferating cell nuclear antigen.
Application : WB
Reactivity : Mouse
Shibata Y et al. J Biol Chem 2002; 277 (1):746-54 Defective flap endonuclease 1 activity in mammalian cells is associated with impaired DNA repair and prolonged S phase delay.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Package List Price ($)
$ 399
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