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Bcl-X antibody

Orthogonal
Anti-Bcl-X antibody used in Western Blot (WB). GTX105661
Anti-Bcl-X antibody used in IHC (Paraffin sections) (IHC-P). GTX105661
Anti-Bcl-X antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105661
Anti-Bcl-X antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105661
Anti-Bcl-X antibody used in Immunoprecipitation (IP). GTX105661
Anti-Bcl-X antibody used in Western Blot (WB). GTX105661
Anti-Bcl-X antibody used in Western Blot (WB). GTX105661
Anti-Bcl-X antibody used in Western Blot (WB). GTX105661
Anti-Bcl-X antibody used in IHC (Paraffin sections) (IHC-P). GTX105661
Anti-Bcl-X antibody used in Western Blot (WB). GTX105661

Cat. No. GTX105661

Reference 13
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Bcl-X products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

NIH-3T3 , PC-12

Predict Reactivity

Rabbit, Sheep, Bovine, Cat, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Bcl-X. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949733

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 like 1 , BCL-XL/S , BCL2L , BCLX , Bcl-X , PPP1R52

Cellular Localization

Mitochondrion membrane; Single-pass membrane protein , Nucleus membrane; Single-pass membrane protein; Cytoplasmic side , Mitochondrion membrane , Nucleus membrane

Background

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Bcl-X antibody used in Western Blot (WB). GTX105661

GTX105661 WB Image

Orthogonal

HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with Bcl-X antibody (GTX105661) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bcl-X antibody used in IHC (Paraffin sections) (IHC-P). GTX105661

GTX105661 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using BCL2L1(GTX105661) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Bcl-X antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105661

GTX105661 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using BCL-x(GTX105661) antibody at 1:500 dilution.

Anti-Bcl-X antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105661

GTX105661 ICC/IF Image

Bcl-X antibody detects Bcl-X protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bcl-X protein stained by Bcl-X antibody (GTX105661) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-Bcl-X antibody used in Immunoprecipitation (IP). GTX105661

GTX105661 IP Image

Immunoprecipitation of Bcl-X protein from HCT-116 whole cell extracts using 5 μg of Bcl-X antibody (GTX105661).
Western blot analysis was performed using Bcl-X antibody (GTX105661) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Bcl-X antibody used in Western Blot (WB). GTX105661

GTX105661 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-X antibody (GTX105661) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bcl-X antibody used in Western Blot (WB). GTX105661

GTX105661 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3
12% SDS PAGE
GTX105661 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bcl-X antibody used in Western Blot (WB). GTX105661

GTX105661 WB Image

Bcl-X antibody detects BCL2L1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
12% SDS-PAGE
Bcl-X antibody (GTX105661) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bcl-X antibody used in IHC (Paraffin sections) (IHC-P). GTX105661

GTX105661 IHC-P Image

Bcl-X antibody detects Bcl-X protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Bcl-X antibody (GTX105661) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Bcl-X antibody used in Western Blot (WB). GTX105661

GTX105661 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-X antibody (GTX105661) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Chiang-Wen Lee et al. Molecules 2022; 27 (2) Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
Application : WB
Reactivity : Human
Lee CW et al. Molecules 2022; 27 (2) : 373 Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
Application : WB
Reactivity : Human
Yi-Chun Chao et al. Int J Mol Sci 2021; 22 (11) Melatonin Downregulates PD-L1 Expression and Modulates Tumor Immunity in KRAS-Mutant Non-Small Cell Lung Cancer.
Application : WB
Reactivity : Human, Mouse
Wang WJ et al. JCI Insight 2021; 6 (15) MCL1 participates in leptin-promoted mitochondrial fusion and contributes to drug resistance in gallbladder cancer.
Application : WB
Reactivity : Human
Lee CW et al. J Cell Physiol 2018; (Epub) Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

Bcl-X antibody Cat. No. GTX105661

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-Bcl-X antibody used in Western Blot (WB). GTX105661
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 0-30 μM cisplatin treatment for 24hr.
Anti-Bcl-X antibody used in Western Blot (WB). GTX105661
Date : Anonymous submitted on 09-Jan-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : OKT3 cells
Amount used : 50μg
Blocking : 5% skim milk, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Submit a Review
Application Reference
Chiang-Wen Lee et al. Molecules 2022; 27 (2) Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
Application : WB
Reactivity : Human
Lee CW et al. Molecules 2022; 27 (2):373 Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
Application : WB
Reactivity : Human
Yi-Chun Chao et al. Int J Mol Sci 2021; 22 (11) Melatonin Downregulates PD-L1 Expression and Modulates Tumor Immunity in KRAS-Mutant Non-Small Cell Lung Cancer.
Application : WB
Reactivity : Human, Mouse
Wang WJ et al. JCI Insight 2021; 6 (15) MCL1 participates in leptin-promoted mitochondrial fusion and contributes to drug resistance in gallbladder cancer.
Application : WB
Reactivity : Human
Lee CW et al. J Cell Physiol 2018; (Epub) Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species.
Application : WB
Reactivity : Human
Lee CF et al. Biomedicine (Taipei) 2018; 8 (3):15 benzyl isothiocyanate (bitc) triggers mitochondria-mediated apoptotic machinery in human *-resistant oral cancer car cells.
Application : WB
Reactivity : Human
Fu K et al. Elife 2016; 5 Sam68/KHDRBS1-dependent NF-κB activation confers radioprotection to the colon epithelium in γ-irradiated mice.
Application : WB
Reactivity : Mouse
Chen MF et al. BMC Cancer 2016; 16:532 Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells.
Application : WB
Reactivity : Human
Fu K et al. Elife 2016; 5 Sam68/KHDRBS1 is critical for colon tumorigenesis by regulating genotoxic stress-induced NF-κB activation.
Application : WB
Reactivity : Mouse
Chen PH et al. Neuropharmacology 2016; 110 (Pt A):362-75 The miR-204-3p-targeted IGFBP2 pathway is involved in xanthohumol-induced glioma cell apoptotic death.
Application : WB
Reactivity : Human
Huang YT et al. Int J Mol Med 2016; 37 (1):83-91 Resveratrol alleviates the cytotoxicity induced by the radiocontrast agent, ioxitalamate, by reducing the production of reactive oxygen species in HK-2 human renal proximal tubule epithelial cells in-vitro.
Wier EM et al. FEBS Lett 2015; 589 (23):614156 Caspase-3 cleaved p65 fragment dampens NF-κB-mediated anti-apoptotic transcription by interfering with the p65/RPS3 interaction.
Application : WB
Reactivity : Human
Moore MJ et al. Cell 2010; 142 (4):625-36 An alternative splicing network links cell-cycle control to apoptosis.
Application : WB
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169