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PGD antibody [N1N3]

Orthogonal
Anti-PGD antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101703
Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703
Anti-PGD antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101703
Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703
Anti-PGD antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101703
Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703
Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703

Cat. No. GTX101703

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PGD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

293T , Molt-4 , Raji , mouse heart , mouse lung

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Chicken, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951203

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphogluconate dehydrogenase , 6PGD

Background

6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PGD antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101703

GTX101703 ICC/IF Image

PGD antibody [N1N3] detects PGD protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGD protein stained by PGD antibody [N1N3] (GTX101703) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703

GTX101703 WB Image

PGD antibody [N1N3] detects PGD protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody [N1N3] (GTX101703) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGD antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101703

GTX101703 ICC/IF Image

PGD antibody [N1N3] detects PGD protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PGD protein stained by PGD antibody [N1N3] (GTX101703) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703

GTX101703 WB Image

Sample(30 μg whole cell lysate)
A:293T
B:MOLT4 (GTX27912)
C:Raji (GTX27908)
10% SDS PAGE
GTX101703 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGD antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101703

GTX101703 IHC-P Image

PGD antibody [N1N3] detects PGD protein at cytoplasm on A549 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded A549 xenograft.
PGD antibody [N1N3] (GTX101703) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703

GTX101703 WB Image

PGD antibody [N1N3] detects PGD protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody [N1N3] (GTX101703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGD antibody [N1N3] used in Western Blot (WB). GTX101703

GTX101703 WB Image

Orthogonal

The data was published in the journal PLoS Biol in 2010.PMID: 21049082

REFERENCE

Application Reference

Choi EJ et al. Oncogene 2017; (Epub) A clinical drug library screen identifies * propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer.
Application : WB
Reactivity : Human
Lemons JM et al. PLoS Biol 2010; 8 (10) : e1000514 Quiescent fibroblasts exhibit high metabolic activity.
Application : WB
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169