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Laforin antibody [C2C3], C-term

Anti-Laforin antibody [C2C3], C-term used in Western Blot (WB). GTX104648
Anti-Laforin antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104648
Anti-Laforin antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104648
Anti-Laforin antibody [C2C3], C-term used in Western Blot (WB). GTX104648

Cat. No. GTX104648

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Laforin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

Raji , 293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Laforin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240770

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EPM2A glucan phosphatase, laforin , EPM2 , MELF

Cellular Localization

Cytoplasm , Isoform 1: Endoplasmic reticulum , Cell membrane , Isoform 2: Endoplasmic reticulum , Nucleus

Background

This gene encodes a dual-specificity phosphatase that associates with polyribosomes. The encoded protein may be involved in the regulation of glycogen metabolism. Mutations in this gene have been associated with myoclonic epilepsy of Lafora. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Laforin antibody [C2C3], C-term used in Western Blot (WB). GTX104648

GTX104648 WB Image

Sample(30 μg of whole cell lysate)
A:Raji(GTX27908)
12% SDS PAGE
GTX104648 diluted at 1:5000

Anti-Laforin antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104648

GTX104648 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using Laforin(GTX104648) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Laforin antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104648

GTX104648 ICC/IF Image

Laforin antibody [C2C3], C-term detects EPM2A protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: EPM2A protein stained by Laforin antibody [C2C3], C-term (GTX104648) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-Laforin antibody [C2C3], C-term used in Western Blot (WB). GTX104648

GTX104648 WB Image

Laforin antibody detects Laforin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Laforin antibody (GTX104648) diluted by 1:3000.

REFERENCE

Application Reference

Dukhande VV et al. PLoS One 2011; 6 (8) : e24040 Laforin, a dual specificity phosphatase involved in Lafora disease, is present mainly as monomeric form with full phosphatase activity.
Application : WB
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169