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SUV39H1 antibody [N3C3]

Anti-SUV39H1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112263
Anti-SUV39H1 antibody [N3C3] used in Western Blot (WB). GTX112263
Anti-SUV39H1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112263

Cat. No. GTX112263

Reference 6
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Monkey
Package
100 μl ($399),
25 μl ($169)
See all SUV39H1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SUV39H1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952113

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

suppressor of variegation 3-9 homolog 1 , H3-K9-HMTase 1 , KMT1A , MG44 , SUV39H

Cellular Localization

Nucleus , Centromere

Background

This gene is a member of the suppressor of variegation 3-9 homolog family and encodes a protein with a chromodomain and a C-terminal SET domain. This nuclear protein moves to the centromeres during mitosis and functions as a histone methyltransferase, methylating Lys-9 of histone H3. Overall, it plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SUV39H1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112263

GTX112263 ICC/IF Image

SUV39H1 antibody [N3C3] detects SUV39H1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SUV39H1 stained by SUV39H1 antibody [N3C3] (GTX112263) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

Anti-SUV39H1 antibody [N3C3] used in Western Blot (WB). GTX112263

GTX112263 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SUV39H1 antibody [N3C3] (GTX112263) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SUV39H1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112263

GTX112263 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using SUV39H1(GTX112263) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Lee JE et al. Commun Biol 2020; 3 (1) : 292 Aging increases vulnerability to stress-induced depression via upregulation of NADPH oxidase in mice.
Reactivity : Mouse
Ishiguro K et al. Clin Immunol 2017; 180 : 120-127 Inhibition of KDM4A activity as a strategy to suppress interleukin-6 production and attenuate colitis induction.
Application : WB
Reactivity : Mouse
Luo CW et al. Radiother Oncol 2017; (Epub) G9a governs colon cancer stem cell phenotype and chemoradioresistance through PP2A-RPA axis-mediated DNA damage response.
Application : WB
Reactivity : Human
Mancini M et al. Toxicol In Vitro 2016; 40 : 43110 In vitro hydroquinone-induced instauration of histone bivalent mark on human retroelements (LINE-1) in HL60 cells.
Application : WB
Reactivity : Human
Imai K et al. FEBS J 2014; Role of the histone H3 lysine-9 methyltransferase Suv39-h1 in maintaining Epsteinn-Barr virus latency in B95-8 cells.
Application : WB
Reactivity : Monkey
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REVIEW

SUV39H1 antibody [N3C3] Cat. No. GTX112263

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-SUV39H1 antibody [N3C3] used in Western Blot (WB). GTX112263
Date : Anonymous submitted on 08-May-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 10 μg
Blocking : 5% skim milk in TBST, 27°C, 1Hr
Primary Antibody : 1:5000, 4°C, 12Hr
Secondary Antibody : GTX213110-01, 1:5000, 27°C, 2Hr
Lane Description : Two groups (Sorafenib -/+) of whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SUV39H1 antibody (GTX112263) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169