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ATG12 antibody [EPR4800], C-term

Anti-ATG12 antibody [EPR4800], C-term used in Western Blot (WB). GTX62935
Anti-ATG12 antibody [EPR4800], C-term used in IHC (Paraffin sections) (IHC-P). GTX62935

Cat. No. GTX62935

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 EPR4800

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all ATG12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol, 0.05% BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues on the C-terminus in Human Apg12.

Purification

Tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2888100

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

autophagy related 12 , APG12 , APG12L , FBR93 , HAPG12

Cellular Localization

Cytoplasm

Background

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-ATG12 antibody [EPR4800], C-term used in Western Blot (WB). GTX62935

GTX62935 WB Image

WB analysis of lysates from (1) Apg12-transfected and (2) non-transfected 293T cells (10 μg per lane) using Apg12 antibody [EPR4800] at a dilution of 1:1,000.

Anti-ATG12 antibody [EPR4800], C-term used in IHC (Paraffin sections) (IHC-P). GTX62935

GTX62935 IHC-P Image

IHC-P analysis of human breast carcinoma using Apg12 antibody [EPR4800] at a dilution of 1:250.

REFERENCE

Application Reference

Chung SD et al. PLoS One 2012; 7 (10) : e47299 Activating Nrf-2 signaling depresses unilateral ureteral obstruction-evoked mitochondrial stress-related autophagy, apoptosis and pyroptosis in kidney.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399