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SNX18 antibody

KO/KD
Anti-SNX18 antibody used in Western Blot (WB). GTX119426
Anti-SNX18 antibody used in IHC (Paraffin sections) (IHC-P). GTX119426
Anti-SNX18 antibody used in Western Blot (WB). GTX119426
Anti-SNX18 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119426
Anti-SNX18 antibody used in Western Blot (WB). GTX119426

Cat. No. GTX119426

Reference 3
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399),
25 μl ($169)
See all SNX18 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

C8D30 , A549 , PC-12

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SNX18. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617597

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sorting nexin 18 , SH3PX2 , SH3PXD3B , SNAG1

Cellular Localization

Endomembrane system; Peripheral membrane protein; Cytoplasmic side

Background

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like some family members, but contains a SH3 domain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SNX18 antibody used in Western Blot (WB). GTX119426

GTX119426 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX119426 diluted at 1:5000

Anti-SNX18 antibody used in IHC (Paraffin sections) (IHC-P). GTX119426

GTX119426 IHC-P Image

Immunohistochemical analysis of paraffin-embedded cal27 xenograft, using SNX18(GTX119426) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SNX18 antibody used in Western Blot (WB). GTX119426

GTX119426 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SNX18 antibody (GTX119426) diluted at 1:1000.

Anti-SNX18 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119426

GTX119426 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SNX18(GTX119426) antibody at 1:200 dilution.

Anti-SNX18 antibody used in Western Blot (WB). GTX119426

GTX119426 WB Image

Sample (30 ug of whole cell lysate)
A: C8D30
7.5% SDS PAGE
GTX119426 diluted at 1:10000

REFERENCE

Application Reference

Wang H et al. J Biol Chem 2020; 295 (4) : 1091-1104 Phosphatidylinositol 3,4-bisphosphate synthesis and turnover are spatially segregated in the endocytic pathway.
Alejandro Martorell Riera et al. bioRxiv 2019; (Epub) Dynamin-2 controls phagophore maturation.
Posor Y et al. Nature 2013; 499 (7457) : 233-7 Spatiotemporal control of endocytosis by phosphatidylinositol-3,4-bisphosphate.
Application : WB
Reactivity : Monkey
Submit a Reference

REVIEW

SNX18 antibody Cat. No. GTX119426

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-SNX18 antibody used in Western Blot (WB). GTX119426
Date : Anonymous submitted on 28-Jan-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Primary Antibody : 1:1000, °C, Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169