GeneTex

  • Country / Location Selection

United States (US)

Pygopus 2 antibody

Anti-Pygopus 2 antibody used in Immunoprecipitation (IP). GTX119726
Anti-Pygopus 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX119726
Anti-Pygopus 2 antibody used in Western Blot (WB). GTX119726
Anti-Pygopus 2 antibody used in Western Blot (WB). GTX119726
Anti-Pygopus 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX119726
Anti-Pygopus 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119726

Cat. No. GTX119726

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Pygopus 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Positive Control

NIH-3T3

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Full length mouse Pygopus 2 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618352

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pygopus 2 , 1190004M21Rik

Database

Research Area

DATA IMAGES

Anti-Pygopus 2 antibody used in Immunoprecipitation (IP). GTX119726

GTX119726 IP Image

GTX119726 IP Image
pygopus 2 was immunoprecipitated from 5*107 cells of SW480 whole cell lysate using 1 ug of anti-pygopus 2 antibody (GTX119726).
The precipitated pygopus 2 was detected by GTX119726 diluted at 1:15000. A: IP-IgG

B: IP-pygopus 2 antibody

Anti-Pygopus 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX119726

GTX119726 IHC-P Image

Pygopus 2 antibody detects Pygopus 2 protein at nucleus in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
Pygopus 2 antibody (GTX119726) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Pygopus 2 antibody used in Western Blot (WB). GTX119726

GTX119726 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Pygopus 2 antibody (GTX119726) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Pygopus 2 antibody used in Western Blot (WB). GTX119726

GTX119726 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Pygopus 2 antibody (GTX119726) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Pygopus 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX119726

GTX119726 IHC-P Image

Pygopus 2 antibody detects Pygopus 2 protein at nucleus in mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Pygopus 2 antibody (GTX119726) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Pygopus 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119726

GTX119726 ICC/IF Image

Pygopus 2 antibody detects Pygopus 2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Pygopus 2 protein stained by Pygopus 2 antibody (GTX119726) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Lu X et al. Cancer Res 2018; (Epub) An in vivo screen identifies PYGO2 as a driver for metastatic prostate cancer.
Yang L et al. Nature 2013; 500 (7464) : 598-602 lncRNA-dependent mechanisms of androgen-receptor-regulated gene activation programs.
Application : WB
Reactivity : Human
Gu B et al. Cell Cycle 2012; 11 (1) : 79-87 Pygo2 regulates histone gene expression and H3 K56 acetylation in human mammary epithelial cells.
Application : ChIP assay
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Pygopus 2 antibody (GTX119726). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169