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Rhodamine antibody [11H10]

Cat. No. GTX29093

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 11H10

Isotype

 IgG1

Application

 WB, ELISA, Control, IHC

Reactivity

 Species independent
Package
100 μg ($399)
See all Rhodamine products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:10000
ELISA 1:20000 - 1:30000
Control Assay dependent
IHC 1:1000 - 1:10000
Not tested in other applications.

Product Note

RHODAMINE Monoclonal Antibody was protein A purified and reacts specifically with Rhodamine and its derivatives. Rhodamine isomer 5 and isomer 6 are reactive as TAMRA, as well as TRITC conjugated proteins. No reaction is observed against Texas Red.

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Anti-RHODAMINE Monoclonal Antibody was produced after repeated immunizations of balb/c mice with rhodamine conjugated KLH.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_385442

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

RHODAMINE Monoclonal Antibody specifically detect dyes in the Rhodamine family. Rhodamine is a family member of the fluorone dyes. Examples are Rhodamine 6G and Rhodamine B. They are often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.

REFERENCE

Application Reference

Gerson JE et al. Mol Neurodegener 2018; 13 (1) : 13 Tau oligomers mediate α-synuclein toxicity and can be targeted by immunotherapy.
Castillo-Carranza DL et al. J Neurosci 2015; 35 (12) : 4857-68 Tau immunotherapy modulates both pathological tau and upstream amyloid pathology in an Alzheimer's disease mouse model.
Application : Control
Castillo-Carranza DL et al. J Neurosci 2014; 34 (12) : 4260-72 Passive Immunization with Tau Oligomer Monoclonal Antibody Reverses Tauopathy Phenotypes without Affecting Hyperphosphorylated Neurofibrillary Tangles.
Application : Control
Castillo-Carranza DL et al. J Alzheimers Dis 2014; Specific Targeting of Tau Oligomers in Htau Mice Prevents Cognitive Impairment and Tau Toxicity Following Injection with Brain-Derived Tau Oligomeric Seeds.
Application : Control
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399