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ALAS-E antibody [N3C3]

Orthogonal
Anti-ALAS-E antibody [N3C3] used in Western Blot (WB). GTX104139
Anti-ALAS-E antibody [N3C3] used in Western Blot (WB). GTX104139
Anti-ALAS-E antibody [N3C3] used in Western Blot (WB). GTX104139

Cat. No. GTX104139

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ALAS-E products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

K562,THP-1

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALAS-E. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949625

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

5'-aminolevulinate synthase 2 , ALAS-E , ALASE , ANH1 , ASB , SIDBA1 , XLDPP , XLEPP , XLSA

Cellular Localization

Mitochondrion matrix

Background

The product of this gene specifies an erythroid-specific mitochondrially located enzyme. The encoded protein catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ALAS-E antibody [N3C3] used in Western Blot (WB). GTX104139

GTX104139 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ALAS-E antibody [N3C3]  (GTX104139) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALAS-E antibody [N3C3] used in Western Blot (WB). GTX104139

GTX104139 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX104139 diluted at 1:1000

Anti-ALAS-E antibody [N3C3] used in Western Blot (WB). GTX104139

GTX104139 WB Image

Orthogonal

The data was published in the journal Front Pharmacol in 2014. PMID: 24860503

REFERENCE

Application Reference

Vercellotti GM et al. Front Pharmacol 2014; 5 : 79 H-ferritin ferroxidase induces cytoprotective pathways and inhibits microvascular stasis in transgenic sickle mice.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169