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C6orf97 antibody

Anti-C6orf97 antibody used in IHC (Paraffin sections) (IHC-P). GTX107144
Anti-C6orf97 antibody used in IHC (Paraffin sections) (IHC-P). GTX107144
Anti-C6orf97 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107144
Anti-C6orf97 antibody used in Western Blot (WB). GTX107144
Anti-C6orf97 antibody used in Western Blot (WB). GTX107144

Cat. No. GTX107144

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all C6orf97 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

A549

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human C6orf97. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036392

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coiled-coil domain containing 170 , C6orf97 , bA282P11.1

Background

The function of this gene and its encoded protein is not known. Several genome-wide association studies have implicated the region around this gene to be involved in breast cancer and bone mineral density, but no link to this specific gene has been found. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-C6orf97 antibody used in IHC (Paraffin sections) (IHC-P). GTX107144

GTX107144 IHC-P Image

C6orf97 antibody detects C6orf97 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
C6orf97 antibody (GTX107144) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-C6orf97 antibody used in IHC (Paraffin sections) (IHC-P). GTX107144

GTX107144 IHC-P Image

C6orf97 antibody detects C6orf97 protein at cytoplasm in human endometrial cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial cancer.
C6orf97 antibody (GTX107144) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C6orf97 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107144

GTX107144 ICC/IF Image

C6orf97 antibody detects C6orf97 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C6orf97 protein stained by C6orf97 antibody (GTX107144) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-C6orf97 antibody used in Western Blot (WB). GTX107144

GTX107144 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX107144 diluted at 1:1000

Anti-C6orf97 antibody used in Western Blot (WB). GTX107144

GTX107144 WB Image

The data was published in the journal Nat Commun in 2014. PMID: 25099679

REFERENCE

Application Reference

Buyukcelebi K et al. Nat Commun 2024; 15 (1) : 1169 Integrating leiomyoma genetics, epigenomics, and single-cell transcriptomics reveals causal genetic variants, genes, and cell types.
Veeraraghavan J et al. Nat Commun 2014; 5 : 4577 Recurrent ESR1-CCDC170 rearrangements in an aggressive subset of oestrogen receptor-positive breast cancers.
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169