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TET1 antibody [GT1462]

Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420
Anti-TET1 antibody [GT1462] used in IHC (Paraffin sections) (IHC-P). GTX627420
Anti-TET1 antibody [GT1462] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627420
Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420
Anti-TET1 antibody [GT1462] used in IHC (Paraffin sections) (IHC-P). GTX627420
Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420
Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420

Cat. No. GTX627420

Host

Mouse

Clonality

Monoclonal

Clone Name

GT1462

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

235 kDa. ( Note )

Positive Control

293T , DDDDK-human TET1-transfected 293T cells , NT2D1 nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_11172316

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tet methylcytosine dioxygenase 1 , CXXC6 , LCX , bA119F7.1

Cellular Localization

Nucleus

Background

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

Database

Research Area

DATA IMAGES

Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420

GTX627420 WB Image

TET1 antibody [GT1462] detects TET1 protein by western blot analysis.
A. 50 μg whole cell lysate/extract from 293T cells transfected with scramble siRNA
B. 50 μg whole cell lysate/extract from TET1-knockdowned 293T cells
6% SDS-PAGE
TET1 antibody [GT1462] (GTX627420) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-TET1 antibody [GT1462] used in IHC (Paraffin sections) (IHC-P). GTX627420

GTX627420 IHC-P Image

TET1 antibody [GT1462] detects TET1 protein at nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
TET1 antibody [GT1462] (GTX627420) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TET1 antibody [GT1462] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627420

GTX627420 ICC/IF Image

TET1 antibody [GT1462] detects TET1 protein at nucleus by immunofluorescent analysis. Sample: TET1-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min. Green: TET1 protein stained by TET1 antibody (GTX627420) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.

Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420

GTX627420 WB Image

NT2D1 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT1462] (GTX627420) diluted at 1:500.

Anti-TET1 antibody [GT1462] used in IHC (Paraffin sections) (IHC-P). GTX627420

GTX627420 IHC-P Image

TET1 antibody [GT1462] detects TET1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
TET1 stained by TET1 antibody [GT1462] (GTX627420) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420

GTX627420 WB Image

TET1 antibody detects TET1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells
5 % SDS-PAGE
TET1 antibody (GTX627420) dilution: 1:500

Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420

GTX627420 WB Image

The data was published in the journal Sci Rep in 2019.PMID: 31235851

REFERENCE

REVIEW

TET1 antibody [GT1462] Cat. No. GTX627420

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 26-Mar-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 50μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Lane Description : A. 50 μg whole cell lysate/extract from 293T cells transfected with scramble siRNA, B. 50 μg whole cell lysate/extract from TET1-knockdowned 293T cells
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations