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ATP6V1A antibody

KO/KD
Anti-ATP6V1A antibody used in Western Blot (WB). GTX110815
Anti-ATP6V1A antibody used in Immunoprecipitation (IP). GTX110815
Anti-ATP6V1A antibody used in Western Blot (WB). GTX110815
Anti-ATP6V1A antibody used in Western Blot (WB). GTX110815
Anti-ATP6V1A antibody used in IHC (Paraffin sections) (IHC-P). GTX110815
Anti-ATP6V1A antibody used in Western Blot (WB). GTX110815

Cat. No. GTX110815

Reference 11

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ATP6V1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATP6V1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949704

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase H+ transporting V1 subunit A , ARCL2D , ATP6A1 , ATP6V1A1 , HO68 , IECEE3 , VA68 , VPP2 , Vma1

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq]

Database

  • Gene ID: 523 ATP6V1A
  • UniProt: P38606 ATP6V1A

Research Area

DATA IMAGES

Anti-ATP6V1A antibody used in Western Blot (WB). GTX110815

GTX110815 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody (GTX110815) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP6V1A antibody used in Immunoprecipitation (IP). GTX110815

GTX110815 IP Image

Immunoprecipitation of ATP6V1A protein from HeLa whole cell extracts using 5 μg of ATP6V1A antibody (GTX110815).
Western blot analysis was performed using ATP6V1A antibody (GTX110815).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ATP6V1A antibody used in Western Blot (WB). GTX110815

GTX110815 WB Image

Sample (20 μg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX110815 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP6V1A antibody used in Western Blot (WB). GTX110815

GTX110815 WB Image

ATP6V1A antibody detects ATP6V1A protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody (GTX110815) diluted by 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP6V1A antibody used in IHC (Paraffin sections) (IHC-P). GTX110815

GTX110815 IHC-P Image

ATP6V1A antibody detects ATP6V1A protein at cytoplasm on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ATP6V1A antibody (GTX110815) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ATP6V1A antibody used in Western Blot (WB). GTX110815

GTX110815 WB Image

The data was published in the journal Front Immunol in 2019.PMID: 31781121

REFERENCE

Application Reference

Ju-Hyun Lee et al. Nat Neurosci 2022; 25 (6) : 688-701 Faulty autolysosome acidification in Alzheimer's disease mouse models induces autophagic build-up of Aβ in neurons, yielding senile plaques.
Azucena Perez-Canamas et al. Brain Commun 2021; 3 (1) : fcaa200 Fronto-temporal dementia risk gene TMEM106B has opposing effects in different lysosomal storage disorders.
Application : WB
Reactivity : Mouse
Yoon SJ et al. Front Immunol 2019; 10 : 2636 Thioredoxin-Interacting Protein Promotes Phagosomal Acidification Upon Exposure to Escherichia coli Through Inflammasome-Mediated Caspase-1 Activation in Macrophages.
Zirngibl RA et al. J Cell Biochem 2019; (Epub) Novel c.G630A TCIRG1 mutation causes aberrant splicing resulting in an unusually mild form of autosomal recessive osteopetrosis.
Application : WB
Reactivity : Human
Deng Y et al. Hum Mol Genet 2018; 27 (10) : 1794-1808 The TMEM127 human tumor suppressor is a component of the mTORC1 lysosomal nutrient-sensing complex.
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Application Reference
Ju-Hyun Lee et al. Nat Neurosci 2022; 25 (6):688-701 Faulty autolysosome acidification in Alzheimer's disease mouse models induces autophagic build-up of Aβ in neurons, yielding senile plaques.
Azucena Perez-Canamas et al. Brain Commun 2021; 3 (1):fcaa200 Fronto-temporal dementia risk gene TMEM106B has opposing effects in different lysosomal storage disorders.
Application : WB
Reactivity : Mouse
Yoon SJ et al. Front Immunol 2019; 10:2636 Thioredoxin-Interacting Protein Promotes Phagosomal Acidification Upon Exposure to Escherichia coli Through Inflammasome-Mediated Caspase-1 Activation in Macrophages.
Zirngibl RA et al. J Cell Biochem 2019; (Epub) Novel c.G630A TCIRG1 mutation causes aberrant splicing resulting in an unusually mild form of autosomal recessive osteopetrosis.
Application : WB
Reactivity : Human
Deng Y et al. Hum Mol Genet 2018; 27 (10):1794-1808 The TMEM127 human tumor suppressor is a component of the mTORC1 lysosomal nutrient-sensing complex.
Hashimoto Y et al. Genes Cells 2018; (Epub) TMEM55B contributes to lysosomal homeostasis and amino acid-induced mTORC1 activation.
Application : WB
Reactivity : Human
Wang A et al. Sci Rep 2017; 7 (1):3005 Activity-independent targeting of mTOR to lysosomes in primary osteoclasts.
Application : WB
Reactivity : Mouse
Matsumoto A et al. Nature 2017; 541 (7636):228-232 mTORC1 and muscle regeneration are regulated by the LINC00961-encoded SPAR polypeptide.
Application : WB
Reactivity : Human
Jewell JL et al. Science 2015; 347 (6218):194-8 Metabolism. Differential regulation of mTORC1 by leucine and glutamine.
Application : WB
Reactivity : Mouse
Henderson MX et al. Acta Neuropathol 2015; Neuronal ceroid lipofuscinosis with DNAJC5/CSPα mutation has PPT1 pathology and exhibit aberrant protein palmitoylation.
Application : WB
Reactivity : Human
Rebsamen M et al. Nature 2015; 519 (7544):477-81 SLC38A9 is a component of the lysosomal amino acid sensing machinery that controls mTORC1.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169