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Filamin B antibody [N1], N-term

KO/KD
Anti-Filamin B antibody [N1], N-term used in Western Blot (WB). GTX101206
Anti-Filamin B antibody [N1], N-term used in Western Blot (WB). GTX101206
Anti-Filamin B antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX101206
Anti-Filamin B antibody [N1], N-term used in Western Blot (WB). GTX101206
Anti-Filamin B antibody [N1], N-term used in Western Blot (WB). GTX101206
Anti-Filamin B antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101206

Cat. No. GTX101206

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Filamin B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

278 kDa. ( Note )

Positive Control

U87-MG , NIH-3T3 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Filamin B. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1240816

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

filamin B , ABP-278 , ABP-280 , AOI , FH1 , FLN-B , FLN1L , LRS1 , SCT , TABP , TAP

Cellular Localization

Isoform 1: Cytoplasm , cell cortex , Cytoplasm , cytoskeleton , myofibril , sarcomere , Z-disk , Isoform 2: Cytoplasm , Isoform 3: Cytoplasm , Isoform 6: Cytoplasm

Background

This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Filamin B antibody [N1], N-term used in Western Blot (WB). GTX101206

GTX101206 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody [N1], N-term (GTX101206) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Filamin B antibody [N1], N-term used in Western Blot (WB). GTX101206

GTX101206 WB Image

Filamin B antibody [N1], N-term detects Filamin B protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell extract
5% SDS-PAGE
Filamin B antibody [N1], N-term (GTX101206) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Filamin B antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX101206

GTX101206 IHC-P Image

Filamin B antibody [N1], N-term detects Filamin B protein at cytoplasm in human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Filamin B antibody [N1], N-term (GTX101206) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Filamin B antibody [N1], N-term used in Western Blot (WB). GTX101206

GTX101206 WB Image

Filamin B antibody detects Filamin B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody (GTX101206) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Filamin B antibody [N1], N-term used in Western Blot (WB). GTX101206

GTX101206 WB Image

Sample (30 μg of whole cell lysate)
A: U87-MG
5% SDS PAGE
GTX101206 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Filamin B antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101206

GTX101206 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Filamin B(GTX101206) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

REFERENCE

Application Reference

Yue Chen et al. Front Cardiovasc Med 2021; 8 : 690846 Downregulation of Filamin a Expression in the Aorta Is Correlated With Aortic Dissection.
Application : WB
Reactivity : Human
Li Y et al. Virol Sin 2018; (Epub) Mechanisms and Effects on HBV Replication of the Interaction between HBV Core Protein and Cellular Filamin B.
Application : WB
Reactivity : Human
Surcel A et al. bioRxiv 2017; (Epub) Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion.
Application : WB
Reactivity : Human
Yang CF et al. Hum Mutat 2017; (Epub) Filamin B Loss-of-Function Mutation in Dimerization Domain Causes Autosomal-Recessive Spondylocarpotarsal Synostosis Syndrome with Rib Anomalies.
Application : WB, ICC/IF
Reactivity : Human
Artym VV et al. J Cell Biol 2015; 208 (3) : 331-50 Dense fibrillar collagen is a potent inducer of invadopodia via a specific signaling network.
Application : ICC/IF
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169