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TFEB antibody

Orthogonal
Anti-TFEB antibody used in Western Blot (WB). GTX33541
Anti-TFEB antibody used in IHC (Paraffin sections) (IHC-P). GTX33541
Anti-TFEB antibody used in IHC (Paraffin sections) (IHC-P). GTX33541
Anti-TFEB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33541
Anti-TFEB antibody used in IHC (Paraffin sections) (IHC-P). GTX33541
Anti-TFEB antibody used in Western Blot (WB). GTX33541

Cat. No. GTX33541

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all TFEB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TFEB (NP_009093.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887718

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transcription factor EB , ALPHATFEB , BHLHE35 , TCFEB

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-TFEB antibody used in Western Blot (WB). GTX33541

GTX33541 WB Image

WB analysis of various sample lysates using GTX33541 TFEB antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-TFEB antibody used in IHC (Paraffin sections) (IHC-P). GTX33541

GTX33541 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX33541 TFEB antibody.
Dilution : 1:100

Anti-TFEB antibody used in IHC (Paraffin sections) (IHC-P). GTX33541

GTX33541 IHC-P Image

IHC-P analysis of human colon tissue using GTX33541 TFEB antibody.
Dilution : 1:100

Anti-TFEB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33541

GTX33541 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX33541 TFEB antibody.
Blue : DAPI
Dilution : 1:100

Anti-TFEB antibody used in IHC (Paraffin sections) (IHC-P). GTX33541

GTX33541 IHC-P Image

IHC-P analysis of rat brain tissue using GTX33541 TFEB antibody.
Dilution : 1:100

Anti-TFEB antibody used in Western Blot (WB). GTX33541

GTX33541 WB Image

Orthogonal

The data was published in the 2022 in Cell Death Dis. PMID: 35444186

REFERENCE

Application Reference

Elisa Bisicchia et al. Cell Death Dis 2022; 13 (4) : 381 Restoration of ER proteostasis attenuates remote apoptotic cell death after spinal cord injury by reducing autophagosome overload.
Application : WB
Reactivity : Rat
Kilpatrick K et al. PLoS One 2015; 10 (3) : e0120819 Genetic and chemical activation of TFEB mediates clearance of aggregated α-synuclein.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399