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TMPRSS11D antibody

Anti-TMPRSS11D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117370
Anti-TMPRSS11D antibody used in Western Blot (WB). GTX117370
Anti-TMPRSS11D antibody used in IHC (Paraffin sections) (IHC-P). GTX117370
Anti-TMPRSS11D antibody used in IHC (Paraffin sections) (IHC-P). GTX117370

Cat. No. GTX117370

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TMPRSS11D products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

A431

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human TMPRSS11D. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163589

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transmembrane serine protease 11D , HAT

Cellular Localization

Cell membrane; Single-pass type II membrane protein , Transmembrane protease , serine 11D catalytic chain: Secreted

Background

This gene encodes a trypsin-like serine protease released from the submucosal serous glands onto mucous membrane. It is a type II integral membrane protein and has 29-38% identity in the sequence of the catalytic region with human hepsin, enteropeptidase, acrosin, and mast cell tryptase. The noncatalytic region has little similarity to other known proteins. This protein may play some biological role in the host defense system on the mucous membrane independently of or in cooperation with other substances in airway mucous or bronchial secretions. [provided by RefSeq]

Database

  • Gene ID: 9407 TMPRSS11D
  • UniProt: O60235 TMPRSS11D

Research Area

DATA IMAGES

Anti-TMPRSS11D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117370

GTX117370 ICC/IF Image

TMPRSS11D antibody detects secreted TMPRSS11D protein by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TMPRSS11D protein stained by TMPRSS11D antibody (GTX117370) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-TMPRSS11D antibody used in Western Blot (WB). GTX117370

GTX117370 WB Image

TMPRSS11D antibody detects TMPRSS11D protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TMPRSS11D antibody (GTX117370) diluted by 1:500.

Anti-TMPRSS11D antibody used in IHC (Paraffin sections) (IHC-P). GTX117370

GTX117370 IHC-P Image

TMPRSS11D antibody detects TMPRSS11D protein at cytoplasm in NCl-H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded NCl-H1299 xenograft.
TMPRSS11D antibody (GTX117370) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TMPRSS11D antibody used in IHC (Paraffin sections) (IHC-P). GTX117370

GTX117370 IHC-P Image

TMPRSS11D antibody detects TMPRSS11D protein at cell membrane and cytoplasm in MCF7 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded MCF7 xenograft.
TMPRSS11D antibody (GTX117370) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Ko Sato et al. J Virol 2021; 95 (21) : e0129621 TMPRSS2 Activates Hemagglutinin-Esterase Glycoprotein of Influenza C Virus.
Application : ICC/IF
Reactivity : Human
Sasaki M. et al. J Virol. 2021; 95 (11) : e00398-21 Host serine proteases TMPRSS2 and TMPRSS11D mediate proteolytic activation and trypsin-independent infection in group A rotaviruses.
Application : WB
Reactivity : Human
Yamaya M et al. Pulm Pharmacol Ther 2015; 33 (Epub) : 66-74 The serine protease inhibitor camostat inhibits influenza virus replication and cytokine production in primary cultures of human tracheal epithelial cells.
Application : ICC/IF
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169