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Nestin antibody [GT623]

Comparison Orthogonal
Anti-Nestin antibody [GT623] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630201
Anti-Nestin antibody [GT623] used in Western Blot (WB). GTX630201
Anti-Nestin antibody [GT623] used in Western Blot (WB). GTX630201

Cat. No. GTX630201

Reference 12

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT623

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Nestin products
PRODUCT

Summary

Nestin antibody recognizes nestin, a type-IV intermediate filament protein that migrates at ~240 kDa on western blot. Nestin is an established marker of mesodermal and neuroectodermal fates, and is expressed in neural stem cells. It is also enriched in endothelial cells, regardless of their proliferative state.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

177 kDa. ( Note )

Positive Control

SK-N-SH , IMR32

Product Note

We do not recommend use of this product for Mouse,Rat samples.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Nestin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888203

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nestin , Nbla00170

Background

Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Nestin antibody [GT623] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630201

GTX630201 ICC/IF Image

Nestin antibody [GT623] detects Nestin protein at cytoskeleton by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in ice-cold MeOH for 5 min.
Green: Nestin stained by Nestin antibody [GT623] (GTX630201) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Nestin antibody [GT623] used in Western Blot (WB). GTX630201

GTX630201 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Nestin antibody [GT623] (GTX630201) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Nestin antibody [GT623] used in Western Blot (WB). GTX630201

GTX630201 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with Nestin antibody [GT623] (GTX630201) diluted at 1:1000 and competitor's antibody (MAB5326) diluted by 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

Application Reference

S Sakatoku et al. Stem Cells Int 2024; : 7685280 Periostin Is a Candidate Regulator of the Host Microenvironment in Regeneration of Pulp and Dentin Complex and Periodontal Ligament in Transplantation with Stem Cell-Conditioned Medium
Application : ICC/IF
Seo Y et al. Int J Mol Sci 2023; 24 (6) Endogenous Neural Stem Cell Activation after Low-Intensity Focused Ultrasound-Induced Blood-Brain Barrier Modulation.
Application : WB
Yao P et al. Folia Histochem Cytobiol 2022; (Epub) Wnt/PCP pathway regulates the migration and neural differentiation of mesenchymal stem cells in vitro.
Ma L et al. J Radiat Res 2022; (Epub) Irradiated microvascular endothelial cells may induce bystander effects in neural stem cells leading to neurogenesis inhibition.
Ming Zhang et al. Nat Commun 2021; 12 (1) : 5091 Ten-eleven translocation 1 mediated-DNA hydroxymethylation is required for myelination and remyelination in the mouse brain.
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REVIEW

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Application Reference
S Sakatoku et al. Stem Cells Int 2024;:7685280 Periostin Is a Candidate Regulator of the Host Microenvironment in Regeneration of Pulp and Dentin Complex and Periodontal Ligament in Transplantation with Stem Cell-Conditioned Medium
Application : ICC/IF
Seo Y et al. Int J Mol Sci 2023; 24 (6) Endogenous Neural Stem Cell Activation after Low-Intensity Focused Ultrasound-Induced Blood-Brain Barrier Modulation.
Application : WB
Yao P et al. Folia Histochem Cytobiol 2022; (Epub) Wnt/PCP pathway regulates the migration and neural differentiation of mesenchymal stem cells in vitro.
Ma L et al. J Radiat Res 2022; (Epub) Irradiated microvascular endothelial cells may induce bystander effects in neural stem cells leading to neurogenesis inhibition.
Ming Zhang et al. Nat Commun 2021; 12 (1):5091 Ten-eleven translocation 1 mediated-DNA hydroxymethylation is required for myelination and remyelination in the mouse brain.
Deng H et al. Cell Transplant 2020; 29:9.6369E+14 Long-Term Liraglutide Administration Induces Pancreas Neogenesis in Adult T2DM Mice.
Koffler J et al. Nat Med 2019; 25 (2):263-269 Biomimetic 3D-printed scaffolds for spinal cord injury repair.
Mansel C et al. Neurotoxicol Teratol 2019; 72:58-70 Lead exposure reduces survival, neuronal determination, and differentiation of P19 stem cells.
Liu H et al. Cell Death Differ 2018; (Epub) Biochemical re-programming of human dermal stem cells to neurons by increasing mitochondrial membrane potential.
Reactivity : Human
Xie MQ et al. Neuroscience 2017; 352:64-78 Newborn dopaminergic neurons are associated with the migration and differentiation of SVZ-derived neural progenitors in a 6-hydroxydopamin-injected mouse model.
Aguilera-Castrejon A et al. Neurochem Res 2016; (Epub) Improved Proliferative Capacity of NP-Like Cells Derived from Human Mesenchymal Stromal Cells and Neuronal Transdifferentiation by Small Molecules.
Reactivity : Human
Pringproa K et al. PLoS One 2015; 10 (8):e0135850 Tropism and Induction of Cytokines in Human Embryonic-Stem Cells-Derived Neural Progenitors upon Inoculation with Highly- Pathogenic Avian H5N1 Influenza Virus.
Reactivity : Human
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations