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PI3 kinase p150 antibody [C1C3]

KO/KD Overexpression
Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953
Anti-PI3 kinase p150 antibody [C1C3] used in Immunoprecipitation (IP). GTX108953
Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953
Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953
Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953
Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953
Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953

Cat. No. GTX108953

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PI3 kinase p150 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

153 kDa. ( Note )

Positive Control

Molt-4 , Raji , 293T

Predict Reactivity

Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PI3 kinase p150. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951244

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoinositide-3-kinase regulatory subunit 4 , VPS15 , p150

Background

Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.

Database

Research Area

DATA IMAGES

Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953

GTX108953 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody [C1C3] (GTX108953) diluted at 1:500.

Anti-PI3 kinase p150 antibody [C1C3] used in Immunoprecipitation (IP). GTX108953

GTX108953 IP Image

Immunoprecipitation of PI3-Kinase p150 protein from HeLa whole cell extracts using 5 μg of PI3-Kinase p150 antibody [C1C3] (GTX108953).
Western blot analysis was performed using PI3-Kinase p150 antibody [C1C3] (GTX108953).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953

GTX108953 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
B: Raji
7.5% SDS PAGE
GTX108953 diluted at 1:1000

Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953

GTX108953 WB Image

KO/KD

The data was published in the journal Nat Commun in 2015. PMID: 26387534

Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953

GTX108953 WB Image

KO/KD

The data was published in the journal Nat Commun in 2015. PMID: 26387534

Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953

GTX108953 WB Image

Overexpression

The data was published in the journal Nat Commun in 2019. PMID: 30952952

Anti-PI3 kinase p150 antibody [C1C3] used in Western Blot (WB). GTX108953

GTX108953 WB Image

The data was published in the journal Nat Commun in 2015. PMID: 26387534

REFERENCE

Application Reference

Alkhoury C et al. Nat Cell Biol 2023; 25 (7) : 975-988 Class 3 PI3K coactivates the circadian clock to promote rhythmic de novo purine synthesis.
Iershov A et al. Nat Commun 2019; 10 (1) : 1566 The class 3 PI3K coordinates autophagy and mitochondrial lipid catabolism by controlling nuclear receptor PPARα.
Application : WB
Reactivity : Mouse
Nemazanyy I et al. Nat Commun 2015; 6 : 8283 class iii pi3k regulates organismal glucose homeostasis by providing negative feedback on hepatic * signalling.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169